Chicken CATH-2 Increases Antigen Presentation Markers on Chicken Monocytes and Macrophages

Author(s): Marina D. Kraaij, Albert van Dijk, Maaike R. Scheenstra, Roel M. van Harten, Henk P. Haagsman, Edwin J.A. Veldhuizen*.

Journal Name: Protein & Peptide Letters

Volume 27 , Issue 1 , 2020

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Abstract:

Background: Cathelicidins are a family of Host Defense Peptides (HDPs), that play an important role in the innate immune response. They exert both broad-spectrum antimicrobial activity against pathogens, and strong immunomodulatory functions that affect the response of innate and adaptive immune cells.

Objective: The aim of this study was to investigate immunomodulation by the chicken cathelicidin CATH-2 and compare its activities to those of the human cathelicidin LL-37.

Methods: Chicken macrophages and chicken monocytes were incubated with cathelicidins. Activation of immune cells was determined by measuring surface markers Mannose Receptor Ctype 1 (MRC1) and MHC-II. Cytokine production was measured by qPCR and nitric oxide production was determined using the Griess assay. Finally, the effect of cathelicidins on phagocytosis was measured using carboxylate-modified polystyrene latex beads.

Results: CATH-2 and its all-D enantiomer D-CATH-2 increased MRC1 and MHC-II expression, markers for antigen presentation, on primary chicken monocytes, whereas LL-37 did not. D-CATH- 2 also increased the MRC1 and MHC-II expression if a chicken macrophage cell line (HD11 cells) was used. In addition, LPS-induced NO production by HD11 cells was inhibited by CATH-2 and D-CATH-2.

Conclusion: These results are a clear indication that CATH-2 (and D-CATH-2) affect the activation state of monocytes and macrophages, which leads to optimization of the innate immune response and enhancement of the adaptive immune response.

Keywords: Host defense peptide, MRC1, antigen presentation, HD11 cells, innate immunity, cathelicidins.

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Article Details

VOLUME: 27
ISSUE: 1
Year: 2020
Page: [60 - 66]
Pages: 7
DOI: 10.2174/0929866526666190730125525
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