Solanesol has antibacterial,anticancer, anti-inflammatory and anti-ulcer biological activities, and is a key intermediate for the synthesis of coenzyme Q10, vitamin K2, and anti-cancer synergist SDB and other ubiquinone drugs. Due to its unique chemical structure, solanesol is currently mainly isolated from Solanaceae plants. Tobacco has the highest content of solanesol compared to other Solanaceae plants.At present, the research on the extraction of solanesol from tobacco focuses on extraction and purification. Starting with the extraction and purification of solanesol, the extraction methods, saponification, separation and purification methods and research progress of solanesol from tobacco at home and abroad in recent years were reviewed. The analyses show that the organic solvent extraction method takes a long time and the extraction rate is low. Modern technology-assisted extraction methods such as ultrasound-assisted extraction, microwave-assisted extraction, and supercritical CO2 extraction cannot only improve the extraction rate but also effectively shorten the extraction time. In order to meet the purity requirements of synthetic drugs, the separation and purification of solanesol from the extracting solution is a crucial step and is also the key to restrict large-scale industrial production of high-purity solanesol. Molecularly imprinted technology has the advantages of small investment, simple operation and large scale production compared with chromatography.Solanesol separated by this method can meet the requirement of synthesizing coenzyme Q10. Nowadays, the synthesis of molecularly imprinted solanesol polymers with better adsorption efficiency is one of the important directions in the future research of solanesol purification.