Background: Lipemia can influence laboratory test results by different mechanisms.
Although the liquid chromatography–tandem mass spectrometry (LC-MS/MS) is considered the
reference method for 25(OH)D3, some compounds (carbohydrate, lipids, proteins, etc.) in the
blood may cause a false result indicating a negative or positive deviation rate from the correct
blood level of the test.
Case Report: In this paper, we report a case of D vitamin intoxication due to a false negative result
caused by lipemia. A young woman with a complaint of pain in multiple joints applied to the
physical therapy clinic and was found to have some cystic bone lesions. She was eventually
diagnosed with DM tip 1, familial hyperlipidemia, and nephrolithiasis. Although she had D vitamin
replacement therapy, low levels of blood 25(OH)D3 concentration, measured by an LC-MS/MS
device, were detected. After blood dilution, a high level of 25(OH)D3 and blood intoxication due
to lipid interference were indicated.
Conclusion: From this case, we can conclude that analytical errors caused by the ingredients of a
blood sample may lead to unnecessary treatment and intoxication. While evaluating the blood
25(OH)D3 levels, clinicians should guard against false-negative results due to interference in
patients with familial hyperlipidemia.