Background: Oxidative stress and chronic hyperglycemia are two major side
effects of type 2 diabetes affecting all cell types including mesenchymal stem cells
(MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial
information for efficient treatment.
Methods: Placental mesenchymal stem cells were treated with various concentrations of
glucose, metformin, rapamycin, and hydrogen peroxide to monitor their viability and cell
cycle distribution. Cellular viability was examined via the MTT assay. Cell cycle distribution
was studied by propidium iodide staining and apoptosis was determined using Annexin Vpropidium
iodide staining and flow cytometry. Involvement of potential signaling pathways
was evaluated by Western blotting for activation of Akt, P70S6K, and AMPK.
Results: The results indicated that high glucose augmented cell viability and reduced
metformin toxic potential. However, the hydrogen peroxide and rapamycin toxicities were
Conclusion: Our findings suggest that high glucose concentration has a major effect on
placental mesenchymal stem cell viability in the presence of rapamycin, metformin and
hydrogen peroxide in culture.