Introduction: In continuation of our efforts to find new antimicrobials, herein we report
the synthesis of various pyrazole, pyrazoline, and pyridine based novel bioactive heterocycles (3a-t).
Methods: Newly synthesized compounds were analysed for their antimicrobial activity. Compounds
3c, 3h, 3i, 3k, 3n, and 3q showed significant antimicrobial activity. Molecular docking
study for the most active analogues against DNA gyrase subunit b (PDB ID: 1KZN) corroborated
well with the observed antimicrobial potency exhibiting significant binding affinity.
Results: Interpretation of the chemical structures reported in this paper was based on IR, 1H NMR,
13C NMR, and mass spectral data.