Background: Diabetes Mellitus (DM) accelerates progress of lung cancer. Hyperglycemia,
a critical feature of DM, promotes lung cancer metastasis. Mogroside V is a triterpenoid glycoside
from Siraitia grosvenorii. Interestingly, mogroside V not only plays an anti-diabetic role, but
also has anti-tumor effects.
Objective: In this study, we investigated the metastatic efficiency of mogroside V in lung cancer
cells cultured in hyperglycemia.
Methods: Two lung cancer cell lines-A549 and H1299 were cultured in normoglycemia (5.5mM
glucose) and hyperglycemia (25mM glucose). Cellular proliferation was tested by MTT, invasion
was examined by transwell assay, migration was measured by wound healing assay, cytoskeleton
was stained by Phalloidin-TRITC and the expressions of EMT markers and Rho-GTPase family protein
were detected by western blot.
Results: Hyperglycemia promoted the invasion and migration of A549 and H1299 cells compared
with normoglycemia. Mogroside V inhibited the hyperglycemia-induced invasion and migration.
Hyperglycemia promoted epithelial-mesenchymal transition (EMT), while mogroside V could reverse
this process through up-regulating E-Cadherin expression and down-regulating N-Cadherin,
Vimentin, Snail expressions. Furthermore, mogroside V fractured microfilaments and reduced Rho
A, Rac1, Cdc42 and p-PAK1 expressions under hyperglycemic conditions.
Conclusion: These results suggest that mogroside V inhibits hyperglycemia-induced lung cancer
cells migration and invasion through reversing EMT and damaging cytoskeleton.