Background: The study was conducted to identify the bacterial strain associated with marine
sponge Hyrtiosaff. erectus collected from the Red Sea coastal water and to assess the utilization of
their secondary metabolites for human benefit as antioxidant, anti-Alzheimer, anti-viral, anticancer and
Methods: After biochemical identification of Pesudomance sp. bacterial strain, the total polyphenol
contents, cytotoxic, antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activity
of the Pesudomance sp. ethyl acetate extract were investigated by applying different biochemical
assays. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant
activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2/-azino-bis
(3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical scavenging activity assays. The cytotoxic effects
were investigated by using the human cancerous cell lines.
Results: The anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activities were determined
using ELISA. Qualitative phytochemical analysis of the Pesudomance sp. extract demonstrated the
presence of a large and diverse group of substances such as alkaloids, carbohydrates, flavonoids, phenols,
terpenoids, saponins, and tannins. The strong antioxidant activity of the Pesudomance sp. extract
was mainly attributed to the protective role of polyphenols against reactive oxygen. It was also observed
that Pesudomance sp. extract possessed significant anti-Alzheimer activity with 94% at 1 mg.
The extract showed also high antiviral activity (90%) using reverse transcriptase enzymes inhibition
assay. The examination of the anticancer activity by applying two experimental models, i.e., PTK and
SHKI cleared out high significant percentages of 76.19 and 83.09 %; respectively.
Conclusion: The anti-inflammatory profiling using TNF, COX1, COX2, IL6 also revealed high antiinflammatory
activity with different metabolic pathway of 62.70, 75.444, 79.27 and 54.15 %; respectively.
The present study concluded that ethyl acetate extract of Pesudomance sp. possessed strong antioxidant,
anti-Alzheimer, and anti-viral, anticancer and anti-inflammatory activities. Further studies
are required to purify the bioactive compounds.