Radioprotective Effect of Gliclazide as an Anti-Hyperglycemic Agent Against Genotoxicity Induced by Ionizing Radiation on Human Lymphocytes

Author(s): Maisa Pouri, Zahra Shaghaghi, Arash Ghasemi, Seyed Jalal Hosseinimehr.

Journal Name: Cardiovascular & Hematological Agents in Medicinal Chemistry

Volume 17 , Issue 1 , 2019

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Abstract:

Objectives: Gliclazide (GL) is widely used to reduce hyperglycaemia in diabetic patients. The aim of this study was to investigate the protective effect of GL against chromosome damage induced by ionizing radiation in human blood lymphocytes.

Methods: In this experimental study, peripheral blood samples were collected from human volunteers and treated with GL at various concentrations (5, 25, 50 or 100 μM) for three hours. Then samples were irradiated to X-ray (1.5 Gy). Blood samples were cultured with mitogenic stimulation. The frequencies of micronuclei in cytokinesis-blocked binucleated lymphocyte were determined in different samples. The antioxidant activities of GL were assayed by two different methods.

Results: GL significantly reduced the percentage of micronuclei in lymphocytes that was irradiated. The maximum radioprotection in reduction of percentage of micronuclei in lymphocytes was observed at 100 μM of GL with 52% of efficacy. GL exhibited excellent free radical scavenging activity and reducing power at a concentration dependent activities.

Conclusion: This data provides that GL is a powerful radioprotective agent that could protect healthy cells against the chromosome damage induced by ionizing radiation through antioxidant activity. The radioprotective effect is new indication of GL for patients' protection against side effect induced by ionizing radiation.

Keywords: Genotoxicity, gliclazide, ionizing radiation, radioprotective, reactive oxygen species.

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Article Details

VOLUME: 17
ISSUE: 1
Year: 2019
Page: [40 - 46]
Pages: 7
DOI: 10.2174/1871525717666190524092918

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