Synergistic Effect of Polyalthia longifolia Leaf and Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) by Microscopic Technique

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Author(s): Balasupramaniam Kirubakari, Yeng Chen, Sreenivasan Sasidharan*.

Journal Name: Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents)

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Background: Polyalthia longifolia is a popular medicinal plant and have been widely used as traditional remedy for centuries in curing of various ailments. The purpose of this study was conducted to determine the in situ antimicrobial synergistic effects between Polyalthia longifolia leaf ethyl acetate fraction (PLEAF) and ampicillin against MRSA local isolate by using modern microscopy technique.

Methods: Hence, the evaluation of synergistic activity of PLEAF and ampicillin against MRSA local isolate was conducted with scanning electron microscopy (SEM).

Results: The combinational effect of PLEAF fraction and ampicillin exhibited a significant antibacterial activity against MRSA. Bacterial cells observations showed invagination, impaired cell division, extensive wrinkles, cell shrinkage, appearance of rougher cell with fibrous matrix and clustered cells which confirmed synergistic effect of PLEAF and ampicillin against MRSA local isolate by SEM.

Conclusion: Conclusively, the in situ SEM observation was proved the synergistic antimicrobial activity between PLEAF fraction and ampicillin to destroy the MRSA resistance bacteria which is an important aspect of PLEAF fraction to be used in the future combinational therapy.

Keywords: Combinational therapy, scanning electron microscopy, Polyalthia longifolia, resistant Staphylococcus aureus, Antibacterial agent, natural products

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(E-pub Ahead of Print)
DOI: 10.2174/1871523018666190522112902

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