In silico T-cell and B-cell Epitope Based Vaccine Design Against Alphavirus Strain of Chikungunya

(E-pub Abstract Ahead of Print)

Author(s): Maharij Haroon Jadoon*, Zainab Rehman*, Areeba Khan*, Muhammad Rizwan, Sajid Khan, Azhar Mehmood, Anum Munir.

Journal Name: Infectious Disorders - Drug Targets

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Background: Chikungunya an arbovirus, is transmitted to humans by the bite of Aedes mosquito. The virus occurrences have been reported in Southeast Asian countries including Pakistan since its isolation. Its symptoms include typical febrile illness and arthralgic syndrome. The virus has not decisively proved to be life-threatening.

Methods: The attempt was to design T-cell and B-cell epitope-based vaccine for Chikungunya. The proteome of chikungunya was retrieved, antigenic proteins identified and T-cell epitopes and B-cell epitopes were predicted. Interacting HLA alleles were identified. The final analysis was done to confirm that predicted T-cell epitopes and B-cell epitopes can be used as a vaccine.

Results: About 32 T-cell epitopes and a 10mer B-cell epitope was identified. Both T-cell and B-cell epitopes demonstrated strong interactions with HLA alleles. The predicted T-cell and B-cell epitopes were docked with respective HLA alleles. The docking analysis showed that the predicted respective epitopes best fit into the binding pockets of the alleles.

Conclusion: On the basis of this computational analysis, it is suggested that these predicted epitopes can be used as a remedy against Alphavirus strain of chikungunya. Further laboratory experiments can be conducted to determine the efficacy and stability of this work.

Keywords: T-cell epitope, B-cell epitope, Chikungunya, HLA allelT-cell epitope, HLA allele

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Article Details

(E-pub Abstract Ahead of Print)
DOI: 10.2174/1871526519666190521100521
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