Expression Profile of Genes Associated with the Proteins Degradation Pathways in Colorectal adenocarcinoma

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Author(s): Martyna Bednarczyk *, Małgorzata Muc-Wierzgoń , Nikola Zmarzły , Beniamin Grabarek, Urszula Mazurek, Grażyna Janikowska.

Journal Name: Current Pharmaceutical Biotechnology

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Changes in the expression of genes associated with the proteins or organelles degradation system in the cell may be a causes or a signal to the carcinogenesis. Thus, the aim of the study was to assess the profile of genes expression linked to the degradation systems of proteins or organelles in histopathologically confirmed colorectal adenocarcinoma in relation to normal colon tissue.

Using oligonucleotide microarrays and the GeneSpring 13.1 and Panther software’s we characterized 1095 mRNAs linked to degradation system of proteins and organelles in sections of colorectal cancer from patients at various clinical stages of disease. In subsequent analyses with restrictive assumptions, we narrowed down the number of genes differentiating cancer, assuming a P-value of less than 0.05. We have found that most of significant changing genes were silenced in all four clinical stages of development of the colorectal cancer.

Only the little-known PTPN22 showed increased expression at all stages. HSPA8 was up-regulated in two early clinical stages (CSI and CSII), and UBB only in the first clinical stage (CSI). Decreased expression of UBC was found in all samples compared to the control tissues.

In summary, examined adenocarcinoma of colorectal cancer is characterized by almost complete silencing of genes related to the degradation of proteins and mitochondria in all clinical stages of disease. The HSPA8 and UBB genes may be potential diagnostic and / or therapeutic targets in the early stages of this cancer.

Keywords: autophagy, cancer, ubiquitination, colon, microarrays, lysosome

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(E-pub Ahead of Print)
DOI: 10.2174/1389201020666190516090744
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