Talazoparib Loaded Solid Lipid Nanoparticles: Preparation, Characterization and Evaluation of the Therapeutic Efficacy In vitro

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Author(s): Gamze Guney Eskiler*, Gulsah Cecener, Gokhan Dikmen, Unal Egeli, Berrin Tunca.

Journal Name: Current Drug Delivery

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Abstract:

In the present work, we report for the first time the therapeutic potential of talazoparib (BMN 673)-SLNs for the treatment of BRCA1 deficient triple negative breast cancer (TNBC). BMN 673-SLNs were produced by hot-homogenization technique then characterized. The cytotoxic and apoptotic effects of BMN 673-SLNs compared with BMN 673 were determined on HCC1937BRCA1-/-, HCC1937-R resistant TNBC and MCF-10A control cell lines. BMN 673-SLNs were found to be a reduced particle size (219.5 ± 1.45 nm) and thus more stable (-28.4 ± 2.52 mV) than BMN 673 (1652 ± 2.46 nm and -18.6 ± 0.45 mV) at 4ºC. In vitro cell line studies demonstrated that BMN 673-SLNs showed significant cytotoxic effects on HCC1937 (29.8%) and HCC1937-R cells (35.7%) at 10 nM for 12 days compared with BMN 673 (HCC1937 cells: 34.0% and HCC1937-R cells: 93.8% at 10 nM for 12 days) (p<0.05). Additionally, BMN 673-SLNs (40.1%) reduced toxicity of BMN 673 (53.1%) on MCF-10A control cells thanks to unique physical properties. The apoptotic rates in the 10 nM BMN 673-SLNs treatment (88.78% and 85.56%) for 12 days were significantly higher than those in 10 nM BMN 673 (82.6% and 25.86%) for 12 days in HCC1937 and HCC1937-R cells, respectively (p<0.01). Furthermore, these effects were consistent with the findings of colony formation, wound healing and calcein accumulation analysis. In conclusion, the therapeutic potential of BMN 673-SLNs provides a promising chemotherapeutic strategy for the treatment of drug-resistant TNBC.

Keywords: Triple negative breast cancer (TNBC), PARP inhibitors, BMN 673 (Talazoparib), Solid Lipid Nanoparticles (SLN), Apoptosis, chemotherapeutic strategy

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(E-pub Ahead of Print)
DOI: 10.2174/1567201816666190515105532
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