Does Nimodipine, a Selective Calcium Channel Blocker, Impair Chondrocyte Proliferation or Damage Extracellular Matrix Structures?

Author(s): Necati Kaplan, Ibrahim Yilmaz, Numan Karaarslan*, Yasin E. Kaya, Duygu Y. Sirin, Hanefi Ozbek.

Journal Name: Current Pharmaceutical Biotechnology

Volume 20 , Issue 6 , 2019

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Graphical Abstract:


Abstract:

Background: The study aimed to investigate the effects of the active ingredient, nimodipine, on chondrocyte proliferation and extracellular matrix (ECM) structures in cartilage tissue cells.

Methods: Chondrocyte cultures were prepared from tissues resected via surgical operations. Nimodipine was then applied to these cultures and molecular analysis was performed. The data obtained were statistically calculated.

Results: Both, the results of the (3-(4,5 dimethylthiazol2-yl)-2,5-diphenyltetrazolium (MTT) assay and the fluorescence microscope analysis [a membrane permeability test carried out with acridine orange/ propidium iodide staining (AO/PI)] confirmed that the active ingredient, nimodipine, negatively affects the cell cultures.

Conclusion: Nimodipine was reported to suppress cellular proliferation; chondroadherin (CHAD) and hypoxia-inducible factor-1 alpha (HIF-1α) expression thus decreased by 2.4 and 1.7 times, respectively, at 24 hrs when compared to the control group (p < 0.05). Furthermore, type II collagen (COL2A1) expression was not detected (p < 0.05). The risk that a drug prescribed by a clinician in an innocuous manner to treat a patient by relieving the symptoms of a disease may affect the proliferation, differentiation, and viability of other cells and/or tissues at the molecular level, beyond its known side effects or adverse events, should not be forgotten.

Keywords: Chondroadherin, chondrocyte, hypoxia-inducible factor-1 alpha, nimodipine, type II collagen, matrix structures.

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Article Details

VOLUME: 20
ISSUE: 6
Year: 2019
Page: [517 - 524]
Pages: 8
DOI: 10.2174/1389201020666190506124548

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