Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

Author(s): Li Li, Jiemin Li, Jian Sun*, Ping Yi, Changbao Li, Zhugui Zhou, Ming Xin, Jinfeng Sheng, Liang Shuai, Zhichun Li, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu, Yayuan Tang.

Journal Name: Current Bioinformatics

Volume 14 , Issue 7 , 2019

Become EABM
Become Reviewer

Graphical Abstract:


Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit.

Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature.

Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit.

Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage.

Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

Keywords: Phospholipase D, postharvest longan, 2-butanol, senescence, gene expression, inhibitor.

Mahankali M, Alter G, Gomez-Cambronero J. Mechanism of enzymatic reaction and protein-protein interactions of PLD from a 3D structural model. Cell Signal 2015; 27: 69-81.
Bargmann BO, Munnik T. The role of phospholipase D in plant stress responses. Curr Opin Plant Biol 2006; 9: 515-22.
Yang N, Yue XL, Chen XL. Molecular cloning and partial characterization of a novel phospholipase D gene from Chorispora bungeana. Plant Cell Tissue Organ Cult 2012; 108: 201-12.
Wang X. The role of phospholipase D in signaling cascades. Plant Physiol 1999; 120: 645-51.
Yang N, Yue XL, Zhang H. Wu GF, Ding FX, Zhang TG,An LZ. Characterization of phospholipase D from Chorispora bungeana callus in response to freezing stress. Biol Plant 2013; 57: 113-20.
Venable ME, Obeid LM. Phospholipase D in cellular senescence. BBA-Mol Cell Biol L 1999; 1439: 291-8.
Yuan H, Chen L, Paliyath G, Sullivan A, Murr DP. Characterization of microsomal and mitochondrial phospholipase D activities and cloning of a phospholipase D alpha cDNA from strawberry fruits. Plant Physiol Biochem 2005; 43: 535-47.
Li M, Hong Y, Wang X. Phospholipase D- and phosphatidic acid-mediated signaling in plants. BBA-Mol Cell Biol L 2009; 1791: 927-35.
Yamada Y, Jinzaki M, Hosokawa T. Molecular structure of phospholipase D and regulatory mechanisms of its activity in plant and animal cells. Biochemistry (Moscow) 2012; 77: 1-14.
Motes CM, Pechter P, Yoo CM, Wang YS, Chapman KD, Blancaflor EB. Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine,on in vivo cytoskeletal organization and Arabidopsis seedling growth. Protoplasma 2005; 226: 109-23.
Peters NT, Logan KO, Miller AC, Kropf DL. Phospholipase D signaling regulates microtubule organization in the fucoid alga Silvetia compressa. Plant Cell Physiol 2007; 48: 1764-74.
Munnik T, Arisz SA, Musgrave VA. G protein activation stimulates phospholipase D signaling in plants. Plant Cell 1995; 7: 2197-210.
Munnik T, Himbergen JA, Riet B. Braun FJ, Irvine RF, Ende H, Musgrave A. Detailed analysis on the turnover of polyphosphoinositides and phosphatidic acid upon activation of phospholipase C and D in Chlamydomonas cells treated with non-permeabilizing concentrations of mastoparan. Planta 1998; 207: 133-45.
Sun J, Zhang E, Xu L, Li Z, Wang Z, Li C. Comparison on characterization of longan (Dimocarpus longan Lour.) polyphenoloxidase using endogenous and exogenous substrates. J Agric Food Chem 2010; 58: 10195-201.
Yang B, Zhao M, Prasad KN, Jiang G, Jiang Y. Effect of methylation on the structure and radical scavenging activity of polysaccharides from longan (Dimocarpus longan Lour.) fruit pericarp. Food Chem 2010; 118: 364-8.
Li L, You XR, Sun J. Peng H, Li C, He QG, Zhang EZ, Liao F, Li ZC. Relationship between gene differential expression of phospholipase D and senescence of plants. J Trop Subtrop Botany 2012; 20: 99-106.
Li L, Li JM, Sun J, et al. Effects of 2-butanol on quality and physiological characteristics of longan fruit stored at ambient temperature. Postharvest Biol Technol 2015; 101: 96-102.
You X, Zhang Y, Li L, et al. Cloning and molecular characterization of phospholipase D (PLD) delta gene from longan (Dimocarpus longan Lour.). Mol Biol Rep 2014; 41: 4351-60.
Jiang Y. Role of anthocyanins, polyphenol oxidase and phenols in lychee pericarp browning. J Sci Food Agric 2000; 80: 305-10.
Jiang Y, Li Y. Effects of chitosan coating on postharvest life and quality of longan fruit. Food Chem 2001; 73: 139-43.
Sun J, You X, Li L, et al. Effects of a phospholipase D inhibitor on postharvest enzymatic browning and oxidative stress of litchi fruit. Postharvest Biol Technol 2011; 62: 288-94.
AOAC. Official Methods of Analysis Association of Official Analytical Chemist. Arlington, VA, USA 2000.
Smith GM, Ward RL, McGuigan L, Rajkovic IA, Scott KF. Measurement of human phospholipase A in arthritis plasma using a newly developed sandwich ELISA. Rheumatology 1992; 31: 175-8.
Yuan H, Chen L, Paliyath G, Sullivan A, Murr DP, Novotna Z. Immunohistochemical localization of phospholipase D in strawberry (Fragaria ananassa Duch.) fruits. Sci Hortic 1939; 109: 35-42.
Liu B, Yao L, Wang W, et al. Molecular cloning and characterization of phospholipase D from Jatropha curcas. Mol Biol Rep 2010; 37: 939-46.
Fan L, Zheng S, Cui D, Wang X. Subcellular distribution and tissue expression of phospholipase Dα, Dβ, and Dγ in Arabidopsis. Plant Physiol 1999; 119: 1371-8.
Christ-Crain M, Kola B, Lolli F, Fekete C. AMP-activated protein kinase mediates glucocorticoid-induced metabolic changes: a novel mechanism in Cushing’s syndrome. The FASEB J 2008; 22: 1672-83.
Nozawa Y. Roles of phospholipase D in apoptosis and pro-survival. BBA-Mol Cell Biol L 2002; 1585: 77-86.
Wei FF. Effect of phospholipase D on induction of cell death, hydrogen peroxide and scopoletin accumulation in tobacco cells treated with ParA1. J Biochem Mol Biol 2010; 26: 949-54.
Laxalt AM, Munnik T. Phospholipid signalling in plant defence. Curr Opin Plant Biol 2002; 5: 332-8.
Xiong L, Schumaker KS, Zhu JK. Cell signaling during cold, drought, and salt stress. Plant Cell 2002; 14: 165-83.
Cristofalo VJ, Lorenzini A, Allen RG, Torres C, Tresini M. Replicative senescence: a critical review. Mech Ageing Dev 2004; 125: 827-48.
Whitaker BD, Smith DL, Green KC. Cloning, characterization and functional expression of a phospholipase Dα cDNA from tomato fruit. Physiol Plant 2010; 112: 87-94.
Ghiso JAA, Alonso DF, Farías EF, Joffé EB. Overproduction of urokinase-type plasminogen activator is regulated by phospholipase D- and protein kinase C-dependent pathways in murine mammary adenocarcinoma cells. BBA-Mol Cell Res 1997; 1356: 171-84.
Kim DU. Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata). BBA-Mol Cell Biol L 1999; 1437: 409-14.
Tiwari K, Paliyath G. Cloning, expression and functional characterization of the C2 domain from tomato phospholipase Dα. Plant Physiol Biochem 2011; 49: 18-32.
Pinhero RG, Almquist KC, Novotna Z, Paliyath G. Developmental regulation of phospholipase D in tomato fruits. Plant Physiol Biochem 2003; 41: 223-40.
Wang X. Phospholipase D in hormonal and stress signaling. Curr Opin Plant Biol 2002; 5: 408-14.
Ryu SB, Wang X. Expression of phospholipase D during castor bean leaf senescence. Plant Physiol 1995; 108: 713-9.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2019
Page: [649 - 657]
Pages: 9
DOI: 10.2174/1574893614666190503162645
Price: $58

Article Metrics

PDF: 22
PRC: 2