Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

Author(s): Li Li, Jiemin Li, Jian Sun*, Ping Yi, Changbao Li, Zhugui Zhou, Ming Xin, Jinfeng Sheng, Liang Shuai, Zhichun Li, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu, Yayuan Tang.

Journal Name: Current Bioinformatics

Volume 14 , Issue 7 , 2019

DOI : 10.2174/1574893614666190503162645

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Graphical Abstract:


Abstract:

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit.

Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature.

Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit.

Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage.

Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

Keywords: Phospholipase D, postharvest longan, 2-butanol, senescence, gene expression, inhibitor.

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Article Details

VOLUME: 14
ISSUE: 7
Year: 2019
Page: [649 - 657]
Pages: 9
DOI: 10.2174/1574893614666190503162645
Price: $58

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