Title:Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit
VOLUME: 14 ISSUE: 7
Author(s):Li Li, Jiemin Li, Jian Sun*, Ping Yi, Changbao Li, Zhugui Zhou, Ming Xin, Jinfeng Sheng, Liang Shuai, Zhichun Li, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu and Yayuan Tang
Affiliation:Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning
Keywords:Phospholipase D, postharvest longan, 2-butanol, senescence, gene expression, inhibitor.
Abstract:
Background: Phospholipase D (PLD)is closely related to browning and senescence of
postharvest longan fruit.
Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and
regulation of PLD during storage of longan fruit at a low temperature.
Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower
pericarp browning index, pulp breakdown index and total soluble solid value than the untreated
fruit.
Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence,
which significantly delayed changes in weight, titratable acidity content, total soluble solid content
and ascorbic acid content. This treatment maintained a high level of total phenolic content and
caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method,
60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results
showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days.
Western-blotting results further confirmed the differential expression of PLD during storage, and a
relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated
fruit during 15-d storage.
Conclusion: These results provided a scientific basis and reference to further investigating postharvest
longan quality maintenance by regulating the PLD gene expression.