Role of phospholipase D inhibitor in regulating expression of senescence-related phospholipase D gene in postharvest longan fruit

(E-pub Abstract Ahead of Print)

Author(s): Li Li, Jiemin Li, Jian Sun*, Changbao Li, Jinfeng Sheng, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu, Yayuan Tang.

Journal Name: Current Bioinformatics

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Phospholipase D (PLD) closely relates to browning and senescence of postharvest longan fruit. This study investigated effects of 2-butanol (a PLD inhibitor) on expression and regulation of PLD during storing longan fruit at low temperature. Senescence-related quality indices showed that 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than untreated fruit. Fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes of weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time quantitative PCR (qRT-PCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further certified the different expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to 2-butanol-treated fruit during 15-d storage. These results provided a scientific basis for further investigating postharvest longan quality maintenance by regulating PLD gene expression.

Keywords: Phospholipase D; postharvest longan; 2-butanol; senescence; gene expression

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Article Details

(E-pub Abstract Ahead of Print)
DOI: 10.2174/1574893614666190503162645
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