Title:Role of phospholipase D inhibitor in regulating expression of senescence-related phospholipase D gene in postharvest longan fruit
VOLUME: 14
Author(s):Li Li, Jiemin Li, Jian Sun*, Changbao Li, Jinfeng Sheng, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu and Yayuan Tang
Affiliation:Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Naning, Guangx, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Naning, Guangxi, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Naning, Guangxi, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Naning, Guangxi, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Naning, Guangxi, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Scien Naning, Guangxi, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Scien Naning, Guangxi, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Scien Naning, Guangxi, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Scien Naning, Guangx, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Scien Naning, Guangx
Keywords:Phospholipase D; postharvest longan; 2-butanol; senescence; gene
expression
Abstract:Phospholipase D (PLD) closely relates to browning and senescence
of postharvest longan fruit. This study investigated effects of 2-butanol (a
PLD inhibitor) on expression and regulation of PLD during storing longan
fruit at low temperature. Senescence-related quality indices showed that
2-butanol-treated fruit presented lower pericarp browning index, pulp
breakdown index and total soluble solid value than untreated fruit. Fruit
treated by 60 µL/L 2-butanol exhibited the strongest inhibition on
senescence, which significantly delayed changes of weight, titratable
acidity content, total soluble solid content and ascorbic acid content. This
treatment maintained a high level of total phenolic content and caused
significant inhibition on pericarp browning and pulp breakdown. Through
ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity.
Real-time quantitative PCR (qRT-PCR) results showed that PLD mRNA expression
level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting
results further certified the different expression of PLD during storage,
and a relatively higher expression for PLD protein was found in control
compared to 2-butanol-treated fruit during 15-d storage. These results
provided a scientific basis for further investigating postharvest longan
quality maintenance by regulating PLD gene expression.
