Background: Many biologically important bioactive compounds were identified in Calophyllum tomentosum extract. Hence, we want to identify different endophytic fungal species from different parts of the plants and their bioactive compounds.
Objective: Identification and characterization of bioactive compounds in extracts of endophytic fungal species of Calophyllum tomentosum.
Methods: Different parts were used to assess the different endophytic fungi. Two different solvents were used extract and qualitative, TLC and GC-MS were employed to identify the bioactive compounds. The bioactive compounds producing endophytes were identified by using18S rRNA.
Results: Totally 13 different fungal species were identified from different parts of Calophyllum tomentosum. The C. tomentosum leaf part had shown seven different fungal endophytes followed by bark (four) and stem (two). The methanol extract of Fusarium species (stem), Cladosporium species (bark) and hexane extract of Alternaria species (bark) have shown more amounts of coumarins in qualitative methods and TLC. Based on the above results, for further GC-MS and molecular identification studies, we have selected three endophytes. The GC-MS analysis, the methanol extract of Fusarium species (stem) shown psoralen, furocoumarin (imperatonin or ammidin), Cladosporium species (bark) shows coumarin (2H-1-benzopyran-2-one), coumaric acid (3-benzofurancarboxylic acid), hynecromone (coumarin 4), 4-hydroxy-9-(3-methyl-2-butyl)furo(3,2-g)chloronen-7-one and hexane extract of Alternaria species (bark) have shown coumaric acid (3-benzofurancarboxylic acid). The three fungal endophytes were identified as Fusarium equiseti, Cladosporium uredinicola and Alternaria alternata from 18S rRNA analysis.
Conclusions: Based on above results the three endophytic fungal species confirms the presence of coumarins and they can use for production of coumarins instead of plant.