MOFs consist of metal containing joint where organic ligands are linked with coordination bonding rendering a unique architecture favoring the attachment of enzymes. MOFs have diverse applications in Chemical catalysis, Gases storage and separation, Luminescence, Magnetism, Molecular sensing, Bio-imaging and also have an impact in drug delivery to the targeted cell/tissue due their The unique properties including ultrahigh porosity, easily tenable, large surface area (up to 6000m2 /g), diverse functionality, and have a very high adsorption capacity. In the past few years immobilization of soluble enzymes on/in MOF has been the topic of interest for scientists working in diverse field. As a potential matrix for immobilization enzymes, the activity of enzyme, reusability, storage, chemical and thermal stability, affinity with substrate can be improved well as compared to other immobilization matrix’s. Along with improvement in enzymes properties, the high loading of enzyme is also observed while using MOFs as immobilization matrix. In this review a detail comparison of immobilization on/in Metal-organic frameworks (MOFs) and classical immobilization techniques have been described. Furthermore, strategies for the enzyme immobilization on MOFs and resulting in improved catalytic performance of immobilized enzymes have been reported.