Age-Related Diazinon Toxicity Impact on Blood Glucose, Lipid Profile and Selected Biochemical Indices in Male Rats

Author(s): Shahnaz Yousefizadeh, Tahereh Farkhondeh, Saeed Samarghandian*.

Journal Name: Current Aging Science

Volume 12 , Issue 1 , 2019

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Graphical Abstract:


Abstract:

Background: Diabetes and its complications are age-related diseases. Low-grade inflammation plays the main role in the aging processes. Diazinon (DZN), an organophosphate pesticide, has been found to induce metabolic disturbances.

Objective: The present study was designed to investigate the impact of DZN on age-related changes on inflammatory cells, blood glucose concentration, lipid profile, and liver and kidney function indices in adult and aged rats.

Methods: Male rats (2 and 16 month old) were orally administrated with DZN (15 mg/kg) for 4 weeks. Then the blood was obtained for measuring inflammatory cells, lipid profile, glucose and serum biochemical indices such as liver enzymes, albumin, total protein, creatinine (Cr), urea, and uric acid in the serum of adult and aged male rats.

Results: DZN increased the blood levels of glucose and the percentage of lymphocytes and also serum levels of TChol, TG, LDL-c, AST, ALT, ALP, LDH, Cr, urea, and uric acid in the adult and aged rats versus the aged matched control rats (p< 0.001). A marked reduction in HDL-c levels, total protein, albumin, and in the percentage of neutrophils were seen in the adult and aged animals exposed to DZN versus the aged matched control rats. DZN also increased the levels of LDL-c and ALT in the aged rats versus adult animals.

Conclusion: The present study indicated that DZN can cause metabolic disturbance. However, the age-dependent effects of DZN on metabolic indices were not be confirmed by the present data.

Keywords: Organophosphate, diazinon, liver, kidney, glucose, lipid profile, aging.

[1]
Lee JS. Cellular senescence, aging, and age-related disease: Special issue of BMB Reports in 2019. BMB Rep 2019; 52(1): 1-2.
[2]
Kong Y, Trabucco SE, Zhang H. Oxidative stress, mitochondrial dysfunction and the mitochondria theory of aging. Interdiscip Top Gerontol 2014; 39: 86-107.
[3]
Gong Z, Tas E, Yakar S, Muzumdar R. Hepatic lipid metabolism and non-alcoholic fatty liver disease in aging. Mol Cell Endocrinol 2017; 455: 115-30.
[4]
Schmitt R, Melk A. Molecular mechanisms of renal aging. Kidney Int 2017; 92(3): 569-79.
[5]
Mehrpour O, Karrari P, Abdollahi M. Chronic lead poisoning in Iran; A silent disease. Daru 2012; 20(1): 8.
[6]
Espandiari P, Zhang J, Schnackenberg LK, Miller TJ, Knapton A, Herman EH, et al. Age-related differences in susceptibility to toxic effects of valproic acid in rats. J Appl Toxicol 2008; 28(5): 628-37.
[7]
Nili-Ahmadabadi A, Alibolandi P, Ranjbar A, Mousavi L, Nili-Ahmadabadi H, Larki-Harchegani A, et al. Thymoquinone attenuates hepatotoxicity and oxidative damage caused by diazinon: An in vivo study. Res Pharm Sci 2018; 13(6): 500-8.
[8]
Mehrpour O, Keyler D, Shadnia S. Comment on Aluminum and Zinc phosphide poisoning. Clin Toxicol 2009; 47(8): 838-9.
[9]
Hernández-Moreno D, Míguez MP, Soler F, Pérez-López M. Influence of sex on biomarkers of oxidative stress in the kidney, lungs, and liver of rabbits after exposure to diazinon. Environ Sci Pollut Res Int 2018; 25(32): 32458-65.
[10]
Gui B, Zhang J, Wang S, Rong G. Anti-osteoarthritic and anti-inflammatory activities of diazine: In vitro and in vivo studies. Med Sci Monit 2018; 24: 76-83.
[11]
Merwin SJ, Obis T, Nunez Y, Re DB. Organophosphate neurotoxicity to the voluntary motor system on the trail of environment-caused amyotrophic lateral sclerosis: The known, the misknown, and the unknown. Arch Toxicol 2017; 91(8): 2939-52.
[12]
Selmi S, Rtibi K, Grami D, Sebai H, Marzouki L. Malathion, an organophosphate insecticide, provokes metabolic, histopathologic and molecular disorders in liver and kidney in prepubertal male mice. Toxicol Rep 2018; 5: 189-95.
[13]
Ramirez-Vargas MA, Flores-Alfaro E, Uriostegui-Acosta M, Alvarez-Fitz P, Parra-Rojas I, Moreno-Godinez ME. Effects of exposure to malathion on blood glucose concentration: A meta-analysis. Environ Sci Pollut Res Int 2018; 25(4): 3233-42.
[14]
Balali-Mood M, Saber H. Recent advances in the treatment of organophosphorous poisonings. Iran J Med Sci 2012; 37(2): 74-91.
[15]
Sams C, Mason HJ, Rawbone R. Evidence for the activation of organophosphate pesticides by cytochromes P450 3A4 and 2D6 in human liver microsomes. Toxicol Lett 2000; 116(3): 217-21.
[16]
Karanth S, Liu J, Ray A, Pope C. Comparative in vivo effects of parathion on striatal acetylcholine accumulation in adult and aged rats. Toxicology 2007; 239(3): 167-79.
[17]
Hariri AT, Moallem SA, Mahmoudi M, Memar B, Razavi BM, Hosseinzadeh H. Effect of Crocus sativus L. stigma (saffron) against subacute effect of diazinon: Histopathological, hematological, biochemical and genotoxicity evaluations in rats. J Pharmacopuncture 2018; 21(2): 61-9.
[18]
El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol 2014; 28(1): 89-93.
[19]
Seals DR, Brunt VE, Rossman MJ. Keynote lecture: Strategies for optimal cardiovascular aging. Am J Physiol Heart Circ Physiol 2018; 315(2): H183-8.
[20]
Janjuha S, Singh SP, Tsakmaki A, Mousavy GSN, Murawala P, Konantz J, et al. Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish. ELife 2018; 7e32965
[21]
Wang N, Ji S, Zhang H, Mei S, Qiao L, Jin X. Herba cistanches: Anti-aging. Aging Dis 2017; 8: 740-59.
[22]
Jung UJ, Choi MS. Obesity and its metabolic complications: The role of adipokines and the relationship between obesity, inflamma-tion, insulin resistance, dyslipidemia and nonalcoholic fatty liver disease. Int J Mol Sci 2014; 15(4): 6184-223.
[23]
Jung UJ, Choi MS. Obesity and its metabolic complications: The role of adipokines and the relationship between obesity, inflammation, insulin resistance, dyslipidemia and nonalcoholic fatty liver disease. Int J Mol Sci 2014; 15(4): 6184-223.
[24]
Yousef MI, el-Demerdash FM, Kamel KI, Al-Salhen KS. Changes in some hematological and biochemical indices of rabbits induced by isoflavones and cypermethrin. Toxicology 2003; 189(3): 223-34.
[25]
Tayeb W, Nakbi A, Trabelsi M, Attia N, Miled A, Hammami M. Hepatotoxicity induced by sub-acute exposure of rats to 2,4-Dichlorophenoxyacetic acid based herbicide “Désormone lourd”. J Hazard Mater 2010; 180(1-3): 225-33.
[26]
El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol 2014; 28(1): 89-93.
[27]
Ben AI, Soudani N, Troudi A, Bouaziz H, Boudawara T, Zeghal N. Antioxidant effect of vitamin E and selenium on hepatotoxicity induced by dimethoate in female adult rats. Ecotoxicol Environ Saf 2011; 74(4): 811-9.
[28]
Vyskočilová E, Szotáková B, Skálová L, Bártíková H, Hlaváčová J, Boušová I. Age-related changes in hepatic activity and expression of detoxification enzymes in male rats. BioMed Res Int 2013; 2013408573
[29]
Khan SS, Singer BD, Vaughan DE. Molecular and physiological manifestations and measurement of aging in humans. Aging Cell 2017; 16(4): 624-33.
[30]
Kalender Y, Uzunhisarcikli M, Ogutcu A, Acikgoz F, Kalender S. Effects of diazinon on pseudocholinesterase activity and haematological indices in rats: The protective role of Vitamin E. Environ Toxicol Pharmacol 2006; 22(1): 46-51.
[31]
Baconi DL, Bârcă M, Manda G, Ciobanu AM, Bălălău C. Investigation of the toxicity of some organophosphorus pesticides in a repeated dose study in rats. Rom J Morphol Embryol 2013; 54(2): 349-56.
[32]
Handy RD, Abd-El Samei HA, Bayomy MF, Mahran AM, Abdeen AM, El-Elaimy EA. Chronic diazinon exposure: Pathologies of spleen, thymus, blood cells, and lymph nodes are modulated by dietary protein or lipid in the mouse. Toxicology 2002; 172(1): 13-34.
[33]
Wang N, Ji S, Zhang H, Mei S, Qiao L, Jin X. Herba cistanches: Anti-aging. Aging Dis 2017; 8(6): 740-59.
[34]
Chang WC, Wu JS, Chen CW, Kuo PL, Chien HM, Wang YT, et al. Protective effect of vanillic acid against hyperinsulinemia, hyperglycemia and hyperlipidemia via alleviating hepatic insulin resistance and inflammation in high-fat diet (HFD)-fed rats. Nutrients 2015; 7(12): 9946-59.
[35]
Lasram MM, Dhouib IB, Annabi A, El Fazaa S, Gharbi N. A review on the molecular mechanisms involved in insulin resistance induced by organophosphorus pesticides. Toxicology 2014; 322: 1-13.


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Article Details

VOLUME: 12
ISSUE: 1
Year: 2019
Page: [49 - 54]
Pages: 6
DOI: 10.2174/1874609812666190416160918

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