Effect of Pullulan on Physicochemical, Microbiological, and Sensory Quality of Yogurts

Author(s): Anna Chlebowska-Śmigiel, Katarzyna Kycia, Katarzyna Neffe-Skocińska, Marek Kieliszek*, Małgorzata Gniewosz, Danuta Kołożyn-Krajewska.

Journal Name: Current Pharmaceutical Biotechnology

Volume 20 , Issue 6 , 2019

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Graphical Abstract:


Background: Pullulan can partially or completely replace starch or fat, thanks to which it can be used for the production of dietetic food. It allows you to maintain the desired consistency, and increases the viscosity of the product.

Objective: Therefore, an attempt was made to produce yogurts with pullulan addition and determine the effect of its presence on the number of LAB, pH change, titratable acidity, and sensory quality of yogurts during storage at 4°C for 28 days.

Methods: The effect of addition of 0; 1.0 and 2.0 % w/v pullulan as a yogurt ingredient on the changes in pH, acidity, LAB number, and sensory quality of yogurt during storage at 4°C for 28 days was examined.

Results: Pullulan did not affect yogurt pH, but the addition of 2.0 % w/v pullulan increased the acidity of yogurt as compared to that of the control yogurt. A statistically significantly higher total number of Lactobacillus, by approximately 1 logarithmic cycle, was found in yogurts with pullulan than in the control yogurt on the 28th day of storage. The yogurt with 1.0% w/v pullulan addition showed better sensory characteristics than that with 2.0% pullulan w/v addition.

Conclusion: The conducted research proved that the presence of pullulan in the culture medium stimulates the growth of selected lactic acid bacteria and influences their fermentation capacity. The use of 1.0 % w/v pullulan during the production of yogurts allowed to obtain a sensorically acceptable product, it had a protective effect on the number of lactic acid bacteria during 28 days of refrigerated storage of yogurt.

Keywords: Pullulan, lactic acid bacteria, Lactobacillus, yogurt, sensory analysis, fermented dairy drinks.

Kycia, K.; Krysiński, C. Microbiological and hygienic quality of commercial goat milk yoghurts in context of their therapeutic properties. Probl. Hig. Epidemiol., 2014, 95(1), 186-191.
Ferdousi, R.; Rouhi, M.; Mohammadi, R.; Mortazavian, A.M.; Khosravi-Darani, K.; Homayouni, R. Evaluation of probiotic survivability in yogurt exposed to cold chain interruption. Iran. J. Pharm. Res., 2013, 12, 139-144.
Neffe, K.; Kołożyn-Krajewska, D. Potential uses of probiotic bacteria in ripening meat products. Zywn-Nauk. Technol. J., 2010, 5(72), 167-177.
Guandalini, S. Are probiotics or prebioticsuseful in pediatric irritable bowel syndrome or inflammatory bowel disease? Front. Med., 2014, 1(23), 1-6.
Gustaw, W.; Kordowska-Wiater, M.; Kozioł, J. The influence of selected prebiotics on the growth of lactic acid bacteria for bio-yoghurt production. Acta Sci. Pol. Technol. Aliment., 2011, 10(4), 455-466.
de Araújo Etchepare, M.; Raddatz, G.C.; de Moraes Flores, E.M.; Queiroz Zepka, L.; Jacob-Lopes, E.; Barin, J.S.; Grosso, C.R.F.; de Menezes, C.R. Effect of resistant starch and chitosan on survival of Lactobacillus acidophilus microencapsulated with sodium alginate. LWT-Food Sci. Technol., 2016, 65, 511-517.
Krasaekoopt, W.; Watcharapoka, S. Effect of addition of inulin and galactooligosaccharide on the survival of microencapsulated probiotics in alginate beads coated with chitosan in simulated digestive system, yogurt and fruit juice. LWT LWT-. Food Sci. Technol, 2014, 57, 761-766.
Yang, L.; Chi, Z.; Wang, G-Y.; Wang, Z-P.; Liu, G-L.; Fu, Ch-F.; Ma, Z-Ch. Taxonomy of Aureobasidium spp. And biosynthesis and regulation of their extracellular polymers. Crit. Rev. Microbiol., 2013, 41(2), 1549-7828.
Zai-Chao, M.; Fu, W-J.; Liu, G-L.; Wang, Z-P.; Chi, Z-M. High-level pullulan production by Aureobasidium pullulans var: omelanogenium P16 isolated from mangrove system. Appl. Microbiol. Biotechnol., 2014, 98, 4865-4873.
Chlebowska-Śmigiel, A.; Gniewosz, M.; Kieliszek, M.; Bzducha-Wróbel, A. The effect of pullulan on the growth and acidifying activity of selected stool microflora of Human. Curr. Pharm. Biotechnol., 2017, 18(2), 121-126.
ISO 7889:2003. Yogurt - Enumeration of characteristic microorganisms. Colony-count technique at 37°C.
QDA ISO 13299.2:1998 Sensory analysis-methodology-general guidance for establishing a sensory profile., ;Geneva, Switzerland:International Organisation of Standardisation.
Gajewska, J.; Błaszczyk, M.K. Probiotic lactic acid bacteria (LAB). Postepy Mikrobiol., 2010, 51(1), 55-65.
Cais-Sokolińska, D.; Michalski, M.; Pikul, J. Role of the proportion of yoghurt bacterial strains in milk souring and the formation of curd qualitative characteristics. Bull. Vet. Inst. Pulawy, 2004, 48, 437-441.
Cais-Sokolińska, D.; Danków, R.; Pikul, J. Dynamics of changes in acidity of yoghurt with an addition of cereals during cold storage. Nauka Przyr. Tech.(Science Nature Technologies)., 2009, 3(4), 1-7.
Stijepic, M.; Glusaci, J.; Pesic-Mikulec, D. Physicochemical characteristics of soy probiotic yoghurt with inulin addition during the refrigerated storage. Rom. Biotechnol. Lett., 2013, 18(2), 102-109.
Tripathi, M.K.; Giri, S.K. Probiotic functional foods: Survival of probiotics during processing and storage. J. Funct. Foods, 2014, 9, 225-241.
Akalin, A.S.; Frenerya, S.; Akbulut, N. Viability and activity of bifidobacteria in yoghurt containing fructooligosaccharide during refrigerated storage. Int. J. Food Sci. Technol., 2004, 39, 613-621.
Pimentel, T.; Garcia, S.; Prudenico, S. Effect of long-chain inulin on the texture profile and survival of Lactobacillus paracasei ssp. paracasei in set yoghurts during refrigerated storage. J. Dairy Technol., 2011, 65(1), 104-111.
Boeni, S.; Pourahmad, R. Use of inulin and probiotic lactobacilli in synbiotic yogurt production. Ann. Biol. Res., 2012, 3(7), 3486-3491.
Gustaw, W.; Nastaj, M.; Sołowiej, B. The effect of hydrocolloids addition on rheological properties of set yoghurt. Zywn-Nauk. Technol. J., 2007, 5(54), 274-282.
Znamirowska, A.; Buniowska, M.; Rożek, P.; Kalicka, D.; Pawlos, M. Evaluation of the quality of thermostatic yoghurts with spelt and inulin. Nauka Przyr. Tech.(Science Nature Technologies)., 2018, 12(1), 103-112.
Kycia, K.; Chlebowska-Śmigiel, A.; Gniewosz, M.; Sokół, E. Effect of pullulan on the physicochemical properties of yoghurt. Int. J. Dairy Technol., 2017, 70(1), 64-70.
Grosso, C.; Fávaro-Trindade, C. Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in Acidified milk and of immobilized B. lactis in yoghurt. Braz. J. Microbiol., 2004, 35, 151-156.

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Article Details

Year: 2019
Page: [489 - 496]
Pages: 8
DOI: 10.2174/1389201020666190416151129
Price: $58

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