Background: Succinic acid can be used as a precursor of many industrially important chemicals including 1,4-butanediol, adipic acid, tetrahydrofuran, γ-butyrolactone, N-methylpyrrolidone, and has an extensive application range as additives for food ingredients, as intermediates for pharmaceutical, as specialty chemicals for detergents, and as precursor for biodegradable polymers such as polybutyrate succinate and polyamides.
Objective: In this work we isolated a strain of B. megaterium which produces succinic acid in czapek medium optimizing the medium and conditions for its production.
Method: A study of the production of this organic acid was realized in different media and adding different insoluble phosphate sources to czapek medium.
Results: Two Bacillus strains ELI 24 and ELI30 that were isolated from soil in Mexico were fully characterized as Bacillus megaterium and B. thuringiensis. B. megaterium ELI 24 was proved to secrete succinic acid which was totally identified by spectroscopic methods, such as 1H and 13C NMR, and X-ray analysis.
Conclusions: This organic acid could improve the solubility of insoluble phosphate, which could help the growth of plants.