Using Cell Cultures for the Investigation of Treatments for Attention Deficit Hyperactivity Disorder: A Systematic Review

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Author(s): Danielly Chierrito, Camila Borges Villas-Boas, Fernanda Stumpf Tonin, Fernando Fernandez-Llimos, Andréia Cristina Conegero Sanches, João Carlos Palazzo Mello*.

Journal Name: Current Neuropharmacology

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Background: Advances in basic and molecular biology have promoted the use of cell cultures in a wide range of areas, including for the evaluation of drug efficacy, safety and toxicity.

Objective: This article was to provide a general overview of the methodological parameters of cell cultures used to investigate therapeutic options for Attention Deficit Hyperactivity Disorder.

Method: A systematic search was performed in the electronic databases PubMed, Scopus, and DOAJ. In vitro experimental studies using cell cultures were included. Results: A total of 328 studies were initially identified, with 16 included for qualitative synthesis. Seven studies used neuronal cells (SH-SY5Y neuroblastoma and PC12 cell line) and nine used non-neuronal cells. All studies described the culture conditions, but most studies were inconsistent with regard to reporting results and raw data. Only one-third of the studies performed cell viability assays, while a further 30% conducted gene expression analysis. Other additional tests included electrophysiological evaluation and transporter activity. More than 50% of the studies evaluated the effects of drugs such as methylphenidate and atomoxetine, while plant extracts were assessed in four studies and polyunsaturated fatty acids in one.

Conclusion: We suggested a flowchart to guide the planning and execution of studies, and a checklist to be completed by authors to allow the standardized reporting of results. This may guide the elaboration of laboratory protocols and further in vitro studies.

Keywords: cell model, neuronal cell, ADHD, ADHD treatment, methodological aspect, flowchart, checklist.

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(E-pub Ahead of Print)
DOI: 10.2174/1570159X17666190409143155
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