Background: Bougainvillea x buttiana has long been used as a Mexican conventional medicinal
drug to cure different sicknesses.
Objective: In this work, the consequences of ethanol combination and temperature extraction for
phenolic compound recovery with the biological activity in Bougainvillea x buttiana extracts were
Materials and Methods: The phenolic recovery from Bougainvillea x buttiana was determined by
comparing the effects of ethanol 0%, 50-100% (v/v) and extraction temperature (26, 45 and 64°C).
The total phenolic content was investigated using spectrophotometric methods. Antioxidant activity
from different Bougainvillea x buttiana extracts was evaluated by determining their potential to scavenge
the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. The chemical compositions and cytotoxic
effect of extracts were determined using GC-MS techniques and the crystal violet technique,
Results: Our results confirmed that all extractions with different concentrations and temperatures had
significant effects over the total phenolic contents and antioxidant activity (DPPH) of the extracts.
The best recuperation of the total phenol content was observed in extracts with 60% ethanol at 26°C
(201.08 mgEGA/g dry extract). Extracts with 100% ethanol at 26°C showed the highest antioxidant
activity (IC50 was 286.75 μg/mL). GC-MS revealed the presence of various phytochemical contents
with polar and non-polar properties. The phenolic compounds were investigated as to the structure,
quantity and position of the hydroxyl groups on the benzene ring.
Conclusion: The ethanolic extract of Bougainvillea x buttiana was confirmed to have high phenolic
content and contain pharmacologically active compounds, with potent antioxidant effects and low cytotoxic