Background: Duck virus hepatitis (DVH) caused by duck hepatitis A virus
type 1 (DHAV-1) is a malignant disease in ducklings, causing economic losses in the
duck industry. However, there is still no antiviral drug against DHAV-1 in the clinic.
Objective: Our aim is to investigate the anti-DHAV-1 effect of baicalin, which is a
flavonoid derived from the Chinese medicinal herb huangqin (Scutellaria baicalensis
Methods: Here, we first detected its anti-DHAV-1 ability in vitro and in vivo. At the same
time, the inhibition of baicalin on DHAV-1 reproduction was determined. Finally, we
tested and verified the anti-oxidative and immuno-enhancing roles of baicalin on its
curative effect on DVH.
Results: Baicalin possessed anti-DHAV-1 effect. It improved the cytoactive of DEH
which was infected by DHAV-1 as well as reduced the DHAV-1 reproduction in DEH.
Under baicalin treatment, mortality of ducklings infected by DHAV-1 decreased,
additionally the DHAV-1 level and liver injury in such ducklings were significantly
reduced or alleviated. The in vitro mechanism study indicated baicalin inhibited DHAV-1
reproduction via interfering the viral replication and release. Furthermore, the in vivo
mechanism study manifested both the anti-oxidative and immuno-enhancing abilities of
baicalin, which played crucial roles in its curative effect on DVH.
Conclusion: This study may provide a scientific basis for developing baicalin as one or
a part of the anti-DHAV-1 drugs.