Introduction: Resveratrol and its monooxygenated metabolite oxyresveratrol were the subject
matter of intense research due to their medicinal value. Absorption, distribution, metabolism and
excretion are important to understand the bioavailability and pharmacokinetic profile of resveratrol and
oxyresveratrol. Quantification of resveratrol and oxyresveratrol is essential for both In Vitro and In
Methods: During In Vitro drug metabolism studies, both qualitative and quantitative information are
essential to understand the metabolic profile of resveratrol and oxyresveratrol. In the present study, a
simple and stable method is outlined using high performance liquid chromatography to quantify both
resveratrol and oxyresveratrol. This method is suitable to understand the metabolic stability, plasma
stability, pharmacokinetics and toxicokinetics of resveratrol and oxyresveratrol.
Results and Conclusion: Generally, In Vitro incubation studies are performed at high concentrations
and In vivo studies are carried out at both high and low concentrations, therefore high performance liquid
chromatography method is demonstrated as a suitable technique to quantify resveratrol and oxyresveratrol.
Retention time of resveratrol and oxyresveratrol from liquid chromatography qualitatively
confirm its identity.