Immunostimulating Activity of Gold-modified Nanodiamond Particles

(E-pub Ahead of Print)

Author(s): Evgenii Plotnikov*, Skoryk Nina, Savelyev Daniil, Vladimir Plotnikov.

Journal Name: Anti-Infective Agents

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Abstract:

Background: Fight against infectious diseases includes two main components – immediate direct anti-infective action and stimulation of one's own immunity.

Objective: In this study, we investigated the properties of diamond nanoparticles modified with gold. The use of such gold nanoparticles as indirect anti-infectious agents and immunostimulators has certain prospects.

Material & methods: Gold hydrosol were synthesized by the reduction in an aqueous solution of gold (III) with sodium citrate (Na3Cit) under heating. Modification procedure of nanodiamond by gold requires incubation a small sample of nanodiamond in a defined volume of gold sol for about 24 hours in a dark place. We use human blood cells as test objects. Reaction of blastic transformation of lymphocytes was applied here as test of biological actions of modified nanodiamond.

Results: Modified nanodiamond do not have toxic influence on blood cells. Modified nanodiamond possess stimulation effects on spontaneous proliferation of lymphocytes and do not significantly affect phytohemagglutinin-induced proliferation. Nanodiamond slightly increase phagocytosis parameters of neutrophil leucocytes.

Conclusion: Thus, results showed that the nanodiamond modified by gold possesses immunostimulating activity, increase in the phagocytic activity of neutrophilic leukocytes and a stimulate lymphocytes in the spontaneous proliferation test. Gold-modified nanodiamond could be considered as non-direct anti-infective agent through immune stimulation.

Keywords: nanodiamond, gold nanoparticle, lymphocytes, phagocytosis, immunostimulation.

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/2211352517666190328122008