Gradual and Asynchronous Increase of Temperature Influence on Insulin Endo- and Exocytosis in Isolated Vertebrata Hepatocytes

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Author(s): Alexander P. Kolychev*, Maxim A. Terpilovskii.

Journal Name: Current Protein & Peptide Science

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Insulin internalization and intracellular insulin/receptor complex (IRC) processing provide with both the hormonal signal transduction elongation into cell and the dissociated complex parts utilization. The patterns of temperature influence on the insulin mechanism of action (IAM), in spite of a general opinion on temperature factor significance, have not been yet revealed. The results of the experimental studies previously carried out on four vertebrate classes isolated hepatocytes (lamprey, frogs, chickens, rats) and the data analysis have revealed the temperature factor particular effects on IAM separate stages in a family of vertebrates and clarified the number of important circumstances. 1- Some essential differentiations of insulin endo and exocytosis activity in poikilothermic and endothermic vertebrate hepatocytes have been found out. 2 - Besides, some significant changes of temperature influence on IAM in a cell have been revealed: a) At the 125 I-insulin internalization high level in cyclostomes (lamprey) hepatocytes the total absence of the process receptibility to temperature has been detected; b) The 125 I-insulin internalization receptibility to temperature at amphibians has been defined but only in the narrow range of low temperatures; c) The medium temperature changes show their influenced on 125 I-insulin internalization in endothermic cells (birds and mammals) within wide range (from 4-to 37ºC). 3 - Besides, the intracellular IRC processing crucial changes in different vertebrate classes hepatocytes have been determined: a) The 125 I-insulin molecule cytosolic degradation so as the 125 I-insulin exocytosis total absence have been revealed in lamprey cells; b) The lysosomal 125 I-insulin degradation takes place with hardly noticeable 125 I-insulin exocytosis in the frog cells; c) The abrupt increase of the degraded fragments portion in the total 125 I- insulin excreted fraction has been defined in chicken and especially in rat cells. Such changes are accompanied the increased temperature influence on the 125 I-insulin separate fractions exocytosis in rat hepatocytes. The data obtained allow us to trace three stages: 1) cyclostomes, 2) amphibians, 3) endothermic (birds, mammals), of temperature influence on 125 I-insulin internalization, and two stages of intracellular IRC processing temperature regulation as well in 1) amphibians, birds, as 2) in mammals. The results of the presented data analysis generally reflect insulin intracellular pathway alterations and hormone utilization during the vertebrata phylogenesis, so the temperature factor has a specific significance in phylogenesis as the selective IAM formation participant.

Keywords: insulin, internalization, endocytosis, exocytosis, degradation, insulin-receptor complex, phylogenesis, temperature, poikilothermic, endodermic, receptibility.

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(E-pub Abstract Ahead of Print)
DOI: 10.2174/1389203720666190325101804
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