Background: Hydrogen has been shown to exert a bioactive effect on the
myocardium. This study examined the signalling pathways for hydrogen attenuating
Methods: In total, 20 male Wistar rats were evaluated for the effects of hydrogen-rich
water on ischaemia-reperfusion in hearts. Left ventricular tissue was taken for screening
and analysis of active protein factors by protein chip technology. The enrichment of the
KEGG pathway was obtained by using the Gene Ontology (GO) enrichment principle.
The expression of JAK2, STAT1, STAT3, p-STAT1, p-JAK2, p-STAT3 in rat myocardium
was detected by Western blot analysis and immunohistochemistry. The apoptosis rates
of the control and hydrogen-rich water groups were detected by TUNEL staining.
Results: The expression levels of 25 proteins, including five transduction pathways,
were downregulated in the hydrogen-rich water group. The expression levels of p-
JAK2/JAK2, p-STAT3/STAT3 were upregulated in the hydrogen-rich water group
compared with the control group, and p-STAT1/STAT1 was downregulated in the
hydrogen-rich water group compared with the control group. Furthermore, the apoptosis
rate was significantly decreased in the hydrogen-rich water group, as well.
Conclusion: Hydrogen-rich water may inhibit the apoptosis of cardiomyocytes after
ischaemia-reperfusion by upregulating the expression of the JAK2-STAT3 signalling
pathway, which reduces ischaemia-reperfusion injury.