Serum Thiol-Disulphide Levels in Epileptic Pediatric Patients

Author(s): Halit Halil*, Nilden Tuygun, Erhan Aksoy, Ozcan Erel, Can Demir Karacan.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 22 , Issue 1 , 2019

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Abstract:

Background: Epilepsy is a serious clinical condition characterized by recurrent seizures. Oxidative stress plays an important role in the etio-pathogenesis of epilepsy. Measurements of serum thiol and disulfide levels were used to evaluate the antioxidant status of the body.

Objective: The aim of this study was to determine serum levels of thiol and disulfide in epileptic pediatric patients.

Methods: Ninety patients, 54 epilepsy and 36 controls were included in the study. Serum levels of native thiol total thiol and disulfide were measured and disulfide/native, disulfide / total thiol and native thiol/ total thiol ratios were calculated. Hence, the ratios of disulfide/ native thiol, disulfide / total thiol and native thiol/ total thiol were calculated.

Results: Serum levels of native thiol, total thiol and disulfide were significantly lower in the epilepsy group than the control group. The ratio of disulfide/native thiol and disulfide / total thiol were significantly higher in the study group than the control group. As well as, the native thiol / total thiol ratio was lower in the epilepsy group than the control group. Native thiol, total thiol and disulfide were significantly lower in the epilepsy group who were taking anti-epileptic drugs than those who were not taking anti-epileptic drugs.

Conclusion: We demonstrated a meaningful relationship between oxidative stress markers and epilepsy in pediatric patients.

Keywords: Biomarkers, disulfide, epilepsy, pediatrics, thiol, oxidative stress.

[1]
Okamoto, K.; Fukuda, M.; Saito, I.; Horiuchi, I.; Okazawa, T.; Ishii, E. Incidence of childhood epilepsy: A population-based study in rural Japan. Brain Dev., 2018, 40(10), 904-908.
[2]
Fisher, R.S.; Acevedo, C.; Arzimanoglou, A.; Bogacz, A.; Cross, J.H.; Elger, C.E. ILAE official report: A practical clinical definition of epilepsy. Epilepsia, 2014, 55, 475-482.
[3]
Stafstrom, C.E.; Carmant, L. Seizures and epilepsy: An overview for neuroscientists. Cold Spring Harb. Perspect. Med., 2015, 5(6), a022426.
[4]
Eldin, E.E.; Elshebiny, H.A.; Mohamed, T.M.; Abdel-Aziz, M.A.; El-Readi, M.Z. The role of antiepileptic drugs in free radicals generation and antioxidant levels in epileptic patients. Int. J. Neurosci., 2016, 126, 105-115.
[5]
Menon, B.; Kumar, R.; Ramalingam, K. Low plasma antioxidant status in patients with epilepsy and the role of antiepileptic drugs on oxidative stress. Ann. Indian Acad. Neurol., 2014, 17, 398-400.
[6]
Ambrogini, P.; Minelli, A.; Galati, C.; Betti, M.; Lattanzi, D.; Ciffolilli, S. Post-seizure α-tocopherol treatment decreases neuroinflammation and neuronal degeneration induced by status epilepticus in rat hippocampus. Mol. Neurobiol., 2014, 50, 246-256.
[7]
dos Santos, P.S.; Costa, J.P.; Tomé Ada, R.; Saldanha, G.B.; de Souza, G.F.; Feng, D.; de Freitas, R.M. Oxidative stress in rat striatum after pilocarpine-induced seizures is diminished by alpha-tocopherol. Eur. J. Pharmacol., 2011, 668, 65-71.
[8]
Boštjan, M.; Iztok, G.; Tomaž, V. Antioxidants as a preventive treatment for epileptic process: A review of the current status. Curr. Neuropharmacol., 2014, 12(6), 527-550.
[9]
Mehvari, J.; Motlagh, F.; Najafi, M.; Ghazvini, M.A.; Naeini, A.; Zare, M. Effects of vitamin E on seizure frequency, electroencephalogram findings, and oxidative stress status of refractory epileptic patients. Adv. Biomed. Res., 2016, 5, 36-38.
[10]
Yürekli, V.A.; Nazıroğlu, M. Selenium and topiramate attenuates blood oxidative toxicity in patients with epilepsy: A clinical pilot study. Biol. Trace Elem. Res., 2013, 152(2), 180-186.
[11]
Erel, O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin. Biochem., 2004, 37, 112-119.
[12]
Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem., 2005, 38, 1103-1111.
[13]
Erenler, A.K.; Yardan, T. Clinical utility of thiol/disulfide homeostasis. Clin. Lab., 2017, 63, 867-870.
[14]
Oliveira, P.V.S.; Laurindo, F.R.M. Implications of plasma thiol redox in disease. Clin. Sci. (Lond.), 2018, 132, 1257-1280.
[15]
Gulpamuk, B.; Tekin, K.; Sonmez, K.; Inanc, M.; Neselioglu, S.; Erel, O.; Yilmazbas, P. The significance of thiol/disulfide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in patients with different stages of diabetes mellitus. Scand. J. Clin. Lab. Invest., 2018, 78(1-2), 136-142.
[16]
Erel, O.; Neselioglu, S. A novel and automated assay for thiol/ disulphide homeostasis. Clin. Biochem., 2014, 47, 326-332.


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Article Details

VOLUME: 22
ISSUE: 1
Year: 2019
Page: [65 - 68]
Pages: 4
DOI: 10.2174/1386207322666190305135043
Price: $58

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