Ultrastructural Analysis of Human Gallstones using Synchrotron Radiation µCT

Author(s): Weixin Chen, Riming Liu, Suo Tao, Weixing Shen, Weihong Zhou, Chao Song, Huanhua Lu, Chungen Xing*.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 22 , Issue 1 , 2019

Submit Manuscript
Submit Proposal

Abstract:

Objective: Gallstone formation is a pathological process of mineralization in the human body. Determination of the morphology and ultrastructure of gallstones holds the key to understanding the pathophysiology of gallbladder disease. Synchrotron radiation phase-contrast Xray microtomography is a novel technology, which is designed for comprehensive analysis of gallstone ultrastructure.

Materials and Methods: Nine human gallstones were obtained from the Department of Pathology, Qingpu branch of Zhongshan Hospital Affiliated to Fudan University (China), and scanned by synchrotron radiation µCT (SR µCT). The imaging data generated by SR µCT scan were analyzed.

Results: The three-dimensional ultrastructure of human gallstones corresponding to their cholesterol and bile pigment composition was determined.

Conclusions: The ultrastructure of gallstones exhibits considerable diversity and complexity. The synchrotron radiation phase-contrast X-ray microtomography is a valuable tool for in-depth study of human gallstones.

Keywords: Gallstone, synchrotron radiation µCT, three-dimensional imaging, ultrastructure, microtomography, novel technology.

[1]
Van Erpecum, K.J. Pathogenesis of cholesterol and pigment gallstones: An update. Clin. Res. Hepatol. Gastroenterol., 2011, 35, 281-287.
[2]
Abeysuriya, V.; Deen, K.I.; Navarathne, N.M. Biliary microlithiasis, sludge, crystals, microcrystallization, and usefulness of assessment of nucleation time. Hepatobiliary Pancreat. Dis. Int., 2010, 9, 248-253.
[3]
Halldestam, I.; Kullman, E.; Borch, K. Incidence of and potential risk factors for gallstone disease in a general population sample. Br. J. Surg., 2009, 96, 1315-1322.
[4]
Ravnborg, L.; Teilum, D.; Pedersen, L.R. Gallbladder stones classified by chemical analysis of cholesterol content. Frederiksberg, 1987-1988. Scand. J. Gastroenterol., 1990, 25, 720-724.
[5]
Sutton, M.D.; Garwood, R.J.; Siveter, D.J. SPIERS and VAXML a software toolkit for tomographic visualisation and a format for virtual specimen interchange. Palaeontol. Electronica, 2012, 2, 396-396.
[6]
Briggs, D.E.; Siveter, D.J.; Siveter, D.J.; Sutton, M.D.; Garwood, R.J.; Legg, D. Silurian horseshoe crab illuminates the evolution of arthropod limbs. Proc. Natl. Acad. Sci. USA, 2012, 109, 15702-15705.
[7]
Sutton, M.D.; Briggs, D.E.; Siveter, D.J.; Siveter, D.J.; Sigwart, J.D. A Silurian armoured aplacophoran and implications for molluscan phylogeny. Nature, 2012, 490, 94-97.
[8]
Huldtgren, T.; Cunningham, J.A.; Yin, C.; Stampanoni, M.; Marone, F.; Donoghue, P.C.; Bengtson, S. Fossilized nuclei and germination structures identify Ediacaran “animal embryos” as encysting protists. Science, 2011, 334, 1696-1699.
[9]
Houssaye, A.; Mazurier, A.; Herrel, A.; Volpato, V.; Tafforeau, P.; Boistel, R.; De Buffrénil, V. Vertebral microanatomy in squamates: Structure, growth and ecological correlates. J. Anat., 2010, 217, 715-727.
[10]
Olejniczak, A.J.; Tafforeau, P.; Smith, T.M.; Temming, H.; Hublin, J.J. Technical note: Compatibility of microtomographic imaging systems for dental measurements. Am. J. Phys. Anthropol., 2007, 134, 130-134.
[11]
Bach-Gansmo, F.L.; Weaver, J.C.; Jensen, M.H.; Leemreize, H.; Mader, K.S.; Stampanoni, M.; Brüel, A.; Thomsen, J.S.; Birkedal, H. Osteocyte lacunar properties in rat cortical bone: Differences between lamellar and central bone. J. Struct. Biol., 2015, 191, 59-67.
[12]
Guan, Y.; Wang, Y.; Yuan, F.; Lu, H.; Ren, Y.; Xiao, T.; Chen, K.; Greenberg, D.A.; Jin, K.; Yang, G.Y. Effect of suture properties on stability of middle cerebral artery occlusion evaluated by synchrotron radiation angiography. Stroke, 2012, 43, 888-891.
[13]
Cariati, A. Gallstone classification in western countries. Indian J. Surg., 2015, 77, 376-380.
[14]
Qiao, T.; Ma, R.H.; Luo, X.B.; Yang, L.Q.; Luo, Z.L.; Zheng, P.M. The systematic classification of gallbladder stones. PLoS One, 2013, 8, e74887.
[15]
Schafmayer, C.; Hartleb, J.; Tepel, J.; Albers, S.; Freitag, S.; Volzke, H.; Buch, S.; Seeger, M.; Timm, B.; Kremer, B.; Fölsch, U.R.; Fändrich, F.; Krawczak, M.; Schreiber, S.; Hampe, J. Predictors of gallstone composition in 1025 symptomatic gallstones from Northern Germany. BMC Gastroenterol., 2006, 6, 36.
[16]
Kim, I.S.; Myung, S.J.; Lee, S.S.; Lee, S.K.; Kim, M.H. Classification and nomenclature of gallstones revisited. Yonsei Med. J., 2003, 44, 561-570.
[17]
Vitek, L.; Carey, M.C. New pathophysiological concepts underlying pathogenesis of pigment gallstones. Clin. Res. Hepatol. Gastroenterol., 2012, 36, 122-129.


Rights & PermissionsPrintExport Cite as


Article Details

VOLUME: 22
ISSUE: 1
Year: 2019
Page: [13 - 17]
Pages: 5
DOI: 10.2174/1386207322666190222122007
Price: $58

Article Metrics

PDF: 29
HTML: 2
EPUB: 1
PRC: 1