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Combinatorial Chemistry & High Throughput Screening

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ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

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Research Article

Ultrastructural Analysis of Human Gallstones using Synchrotron Radiation µCT

Author(s): Weixin Chen, Riming Liu, Suo Tao, Weixing Shen, Weihong Zhou, Chao Song, Huanhua Lu and Chungen Xing*

Volume 22, Issue 1, 2019

Page: [13 - 17] Pages: 5

DOI: 10.2174/1386207322666190222122007

Price: $65

Abstract

Objective: Gallstone formation is a pathological process of mineralization in the human body. Determination of the morphology and ultrastructure of gallstones holds the key to understanding the pathophysiology of gallbladder disease. Synchrotron radiation phase-contrast Xray microtomography is a novel technology, which is designed for comprehensive analysis of gallstone ultrastructure.

Materials and Methods: Nine human gallstones were obtained from the Department of Pathology, Qingpu branch of Zhongshan Hospital Affiliated to Fudan University (China), and scanned by synchrotron radiation µCT (SR µCT). The imaging data generated by SR µCT scan were analyzed.

Results: The three-dimensional ultrastructure of human gallstones corresponding to their cholesterol and bile pigment composition was determined.

Conclusions: The ultrastructure of gallstones exhibits considerable diversity and complexity. The synchrotron radiation phase-contrast X-ray microtomography is a valuable tool for in-depth study of human gallstones.

Keywords: Gallstone, synchrotron radiation µCT, three-dimensional imaging, ultrastructure, microtomography, novel technology.

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