Genetic Diversity of Blattella germanica Isolates from Central China based on Mitochondrial Genes

Author(s): Pan Wei, XiaoDong Xie, Ran Wang, JianFeng Zhang, Feng Li, ZhaoPeng Luo, Zhong Wang, MingZhu Wu, Jun Yang, PeiJian Cao*.

Journal Name: Current Bioinformatics

Volume 14 , Issue 7 , 2019

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Graphical Abstract:


Background: Blattella germanica is a widespread urban invader insect that can spread numerous types of human pathogens, including bacteria, fungi, and protozoa. Despite the medical significance of B. germanica, the genetic diversity of this species has not been investigated across its wide geographical distribution in China.

Objective: In this study, the genetic variation of B. germanica was evaluated in central China.

Methods: Fragments of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the 16S rRNA gene were amplified in 36 B. germanica isolates from 7 regions. The sequence data for COI and 16S rRNA genes were analyzed using bioinformatics methods.

Results: In total, 13 haplotypes were found among the concatenated sequences. Each sampled population, and the total population, had high haplotype diversity (Hd) that was accompanied by low nucleotide diversity (Pi). Molecular genetic variation analysis indicated that 84.33% of the genetic variation derived from intra-region sequences. Phylogenetic analysis indicated that the B. germanica isolates from central China should be classified as a single population. Demographic analysis rejected the hypothesis of sudden population expansion of the B. germanica population.

Conclusion: The 36 isolates of B. germanica sampled in this study had high genetic variation and belonged to the same species. They should be classified as a single population. The mismatch distribution analysis and BSP analysis did not support a demographic population expansion of the B. germanica population, which provided useful knowledge for monitoring changes in parasite populations for future control strategies.

Keywords: Blattella germanica, COI, 16S rRNA, genetic variation, phylogeny, mitochondrial.

Vargo EL, Crissman JR, Booth W, et al. Hierarchical genetic analysis of German cockroach (Blattella germanica) populations from within buildings to across continents. PLoS One 2014; 9e102321
Mukha DV, Kagramanova AS, Lazebnaya IV, et al. Intraspecific variation and populationstructure of the German cockroach, Blattella germanica, revealed with RFLP analysis of the non-transcribed spacer region of ribosomal DNA. Med Vet Entomol 2007; 21: 132-40.
Graczyk TK, Knight R, Tamang L. Mechanical transmission of human protozoan parasites by insects. Clin Microbiol Rev 2005; 18: 128-32.
Pai HH, Chen WC, Peng CF. Isolation of bacteria with antibiotic resistance from household cockroaches (Periplaneta americana and Blattella germanica). Acta Trop 2005; 93: 259-65.
Rosenstreich DL, Eggleston P, Kattan M, et al. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. N Engl J Med 1997; 336: 1356-63.
Hulme PE. Trade, transport and trouble: managing invasive species pathways inan era of globalization. J Appl Ecol 2009; 46: 10-8.
Zink RM, Barrowclough GF. Mitochondrial DNA under siege in avian phylogeography. Mol Ecol 2008; 17: 2107-21.
Makhawi AM, Liu XB, Yang SR, et al. Genetic variations of ND5 gene of mtDNA in populations of Anopheles sinensis (Diptera: Culicidae) malaria vector in China. Parasit Vectors 2013; 6: 290.
Von Beeren C, Stoeckle MY, Xia J, et al. Interbreeding among deeply divergent mitochondrial lineages in the American cockroach (Periplaneta americana). Sci Rep 2015; 5: 8297.
Zhang X, Wang H, Cui J, et al. Characterisation of the relationship between Spirometra erinaceieuropaei and Diphyllobothrium species using complete cytb and cox1 genes. Infect Genet Evol 2015; 35: 1-8.
Yilmaz E, Fritzenwanker M, Pantchev N, et al. The Mitochondrial Genomes of the Zoonotic Canine Filarial Parasites Dirofilaria (Nochtiella) repens and Candidatus Dirofilaria (Nochtiella) Honkongensis Provide Evidence for Presence of Cryptic Species. PLoS Negl Trop Dis 2016; 10e0005028
Folmer O, Black M, Hoeh W, et al. DNA Primers foramplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 1994; 3: 294-9.
Nagaraja Nagaraju J, Ranganath HA. Molecular phylogeny of the nasuta subgroup of Drosophila based on 12S rRNA, 16S rRNA and CoI mitochondrial genes, RAPD and ISSR polymorphisms. Genes Genet Syst 2004; 79: 293-9.
Larkin MA, Blackshields G, Brown NP, et al. Clustal W and clustal X version 2.0. Bioinformatics 2007; 23: 2947-8.
Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731-9.
Xia X, Xie Z. DAMBE: software package for data analysis in molecular biology and evolution. J Hered 2001; 92: 371-3.
Xia X, Xie Z, Salemi M, et al. An index of substitution saturation and its application. Mol Phylogenet Evol 2003; 26: 1-7.
Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 2009; 25: 1451-2.
Posada D. jModelTest: Phylogenetic model averaging. Mol Biol Evol 2008; 25: 1253-6.
Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19: 1572-4.
Bandelt HJ, Forster P, Röhl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 1999; 16: 37-48.
Excoffier L, Lischer HEL. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 2010; 10: 564-7.
Slatkin M. A measure of population subdivision based on microsatellite allele frequencies. Genetics 1995; 139: 457-62.
Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 1989; 123: 585-95.
Fu YX. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 1997; 147: 915-25.
Drummond AJ, Rambaut A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 2007; 7: 214.
Vrsansky P. Mesozoic relative of the common synanthropic German cockroach (Blattodea). Dtsch Entomol Z 2008; 55: 215-21.
Zhang X, Shi YL, Wang ZQ, et al. Morphological and mitochondrial genomic characterization of eyeworms (Thelazia callipaeda) from clinical cases in central China. Front Microbiol 2017; 8: 1335.
Yue Q, Wu K, Qiu D, et al. A formal re-description of the cockroach hebardina concinna anchored on DNA barcodes confirms wing polymorphism and identifies morphological characters for field identification. PLoS One 2014; 9e106789
Neigel JE, Avise JC. Application of a random walk model to geographic distributions of animal mitochondrial DNA variation. Genetics 1993; 135: 1209-20.
Xiao B, Chen AH, Zhang YY, et al. Complete mitochondrial genomes of two cockroaches, Blattella germanica and Periplaneta americana, and the phylogenetic position of termites. Curr Genet 2012; 58: 65-77.
Mao M, Liu HL. Genetic diversity of Trichomonas vaginalis clinical isolates from Henan province in central China. Pathog Glob Health 2015; 109: 242-6.
Balloux F, Lugon-Moulin N. The estimation of population differentiation with microsatellite markers. Mol Ecol 2002; 11: 155-65.
Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 1992; 9: 552-69.
Zhang X, Wang H, Cui J, et al. The phylogenetic diversity of Spirometra erinaceieuropaei isolates from southwest China revealed by multi genes. Acta Trop 2016; 156: 108-14.
Meiklejohn CD, Montooth KL, Rand DM. Positive and negative selection on the mitochondrial genome. Trends Genet 2007; 23: 259-63.

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Article Details

Year: 2019
Page: [574 - 580]
Pages: 7
DOI: 10.2174/1574893614666190204153041
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