Exploring the Patent Landscape of RNAi-based Innovation for Plant Breeding

Author(s): Dario Gianfranco Frisio*, Vera Ventura.

Journal Name: Recent Patents on Biotechnology

Volume 13 , Issue 3 , 2019

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Graphical Abstract:


Background: RNA interference (RNAi) is an innovative technique for plant improvement based on naturally occurring mechanisms which show great potential because of their high specificity and possibility to be applied through innovative methods of topical application in plants. This specific innovation sector is worth analysing from the economic perspective given the great potentiality of RNAi-based plants and products to support modern agriculture in reaching the goals for the improvement of agri-food chains global sustainability. This paper aims to evaluate the global landscape of RNAi innovation by analysing patent data as indicators of innovation output.

Methods: We revised all patents relating to RNAi in plants based on a dataset of roughly seven thousand patent families. The analysis classified inventions according to a set of variables able to characterise the dynamics of innovation (i.e. public/private ownership, type of plants involved, main traits) while the use of concentration indices provided insights into the evolution of this sector.

Results: Results revealed that RNAi is a technique with promising future applications, able to provide solutions to a great variety of agricultural issues and principally developed by the US and Chinese applicants, whereas European innovation capacity in this field appears to be limited.

Conclusion: The innovation landscape of plant breeding is rapidly evolving and RNAi technique is probably going to play a major role in this field.

Keywords: RNAi, new breeding technologies, innovation, patent, plant, agriculture.

Younis A, Siddique MI, Kim CK, Lim KB. RNA interference (RNAi) induced gene silencing: a promising approach of hi-tech plant breeding. Int J Biol Sci 2014; 10: 1150.
Pathak K, Gogoi B. RNA interference (RNAi), application in crop improvement: a review. Agric Rev 2016; 37(3): 245-9.
Borel B. When the pesticides run out. Nat 2017; 543: 302-4.
Majumdar R, Rajasekaran K, Cary JW. RNA Interference (RNAi) as a potential tool for control of mycotoxin contamination in crop plants: concepts and considerations. Front Plant Sci 2017; 8: 200.
Andersen MM, Landes X, Xiang W, et al. Feasibility of new breeding techniques for organic farming. Trends Plant Sci 2015; 20(7): 426-34.
Grishok A, Pasquinelli AE, Conte D, et al. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 2001; 106(1): 23-34.
Uluisik S, Chapman NH, Smith R, et al. Genetic improvement of tomato by targeted control of fruit softening. Nat Biotechnol 2016; 34(9): 950.
Gordon KH, Waterhouse PM. RNAi for insect-proof plants. Nat Biotechnol 2007; 25(11): 1231.
Gheysen G, Vanholme B. RNAi from plants to nematodes. Trends Biotechnol 2007; 25(3): 89-92.
Pooggin M, Shivaprasad PV, Veluthambi K, Hohn T. RNAi targeting of DNA virus in plants. Nat Biotechnol 2003; 21(2): 131.
Huvenne H, Smagghe G. Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. J Insect Physiol 2010; 56(3): 227-35.
Killiny N, Hajeri S, Tiwari S, Gowda S, Stelinski LL. Double-stranded RNA uptake through topical application, mediates silencing of five CYP4 genes and suppresses insecticide resistance in Diaphorina citri. PLoS One 2014; 9(10)110536
Wang M, Jin H. Spray-induced gene silencing: a powerful innovative strategy for crop protection. Trends Microbiol 2017; 25(1): 4-6.
Cai Q, He B, Kogel KH, Jin H. Cross-kingdom RNA trafficking and environmental RNAi-nature’s blueprint for modern crop protection strategies. Curr Opin Microbiol 2018; 46: 58-64.
Wang M, Thomas N, Jin H. Cross-kingdom RNA trafficking and environmental RNAi for powerful innovative pre-and post-harvest plant protection. Curr Opin Plant Biol 2017; 38: 133-41.
Shew AM, Danforth DM, Nalley LL, Nayga Jr R.M., Tsiboe F, Dixon BL. New innovations in agricultural biotech: consumer acceptance of topical RNAi in rice production. Food Control 2017; 81: 189-95.
Malyska A, Bolla R, Twardowski T. The role of public opinion in shaping trajectories of agricultural biotechnology. Trends Biotechnol 2016; 34(7): 530-4.
Palmgren MG, Edenbrandt AK, Vedel SE, et al. Are we ready for back-to-nature crop breeding? Trends Plant Sci 2015; 203: 155-64.
Shew AM, Nalley LL, Danforth DM, et al. Are all GMO s the same? Consumer acceptance of cisgenic rice in India. Plant Biotechnol J 2016; 14(1): 4-7.
Waltz E. Nonbrowning GM apple cleared for market. Nat Biotechnol 2015; 33: 326-7.
Waltz E. USDA approves next-generation GM potato. Nat Biotechnol 2015; 33: 12-3.
Bonny S. Corporate concentration and technological change in the global seed industry. Sustainability 2017; 9(9): 1632.
Lundin P. Is silence still golden? Mapping the RNAi patent landscape Nat Biotechnol 2011; 29.6: 493.
Graff GD, Cullen SE, Bradford KJ, Zilberman D, Bennett AB. The public-private structure of intellectual property ownership in agricultural biotechnology. Nat Biotechnol 2003; 21(9): 989-95.
Chi-Ham CL, Clark KL, Bennett AB. The intellectual property landscape for gene suppression technologies in plants. Nat Biotechnol 2010; 28(1): 32.
Lusser M, Parisi C, Plan D, Rodríguez-Cerezo E. Deployment of new biotechnologies in plant breeding. Nat Biotechnol 2012; 30(3): 231.
Liston-Heyes C, Pilkington A. Inventive concentration in the production of green technology: a comparative analysis of fuel cell patents. Sci Public Policy 2004; 31(1): 15-25.
Wang YH, Luo GL, Guo YW. Why is there overcapacity in China’s PV industry in its early growth stage? Renew Energy 2014; 72: 188-94.
Frisio DG, Ferrazzi G, Ventura V, Vigani M. Public vs private agbiotech research in the United States and European Union, 2002-2009. AgBioForum 2010; 13(4): 333-42.
Naldi M, Flamini M. The CR4 index and the interval estimation of the Herfindahl-Hirschman Index: an empirical comparison 2014.
Rhoades SA. The herfindahl-hirschman index. Fed Reserve Bull 1993; 79: 188.
USDA. China Moving Towards Commercialization of Its Own Biotechnology Crops Gain Report 2017; CH16065.
Han F, Shelton AM, Zhou D. How China can enhance adoption of biotech crops. Nat Biotechnol 2016; 34(7): 693.
Huang J, Yang G. Understanding recent challenges and new food policy in China. Glob Food Secur 2017; 12: 119-26.
James C. Global status of commercialized Biotech/ GM crops: 2016; ISAAA Brief 52.
Guohua L, Yong Z, Huan Z. Method for culturing stripe disease resistant rice by using RNAi technology. CN2011187225. 2011.
Rotenberg D, Whitfield AE, Bockus WW, Chumley FG, Acosta-Leal R. Multigenic transgenic resistance to cereal viruses by rna-interference. WO2017192857. 2017.
Chen W, Fan X, Liu L, Wang K. Method for improving content of lysine in maize by using zein gene ribonucleic acid interference (RNAi) vector. CN20121010544. 2012.
Ren P, Huang X, Chaudhuri S, Talton L, Mcmillan J. Compositions and methods using RNA interference for control of nematodes in plants. WO2005US05756. 2005.
Xiaoya C, Yingbo M, Zhiping L, Lingjian W. Method for modifying insect resistance of plants by utilizing rnai technique. US2010005 0294A1. 2010.

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Article Details

Year: 2019
Page: [207 - 216]
Pages: 10
DOI: 10.2174/1872208313666190204121109
Price: $65

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