Background: Plant mediated biological synthesis is one of the green approaches that has
attracted the attention of the researchers.
Objective: This experiment revealed the biological synthesis of palladium nanoparticles (PdNPs) using
aqueous bark extract of the plant named Bauhinia variegata(Kaanchnar).
Method: Biomolecules present in the plants act as a reducing agent as well as capping agent by which
PdII metal gets reduced, leading to the formation of PdNPs. XRD revealed the crystalline PdNPs posses
face-centered cubic structure with an average size about 2.87 nm. FTIR showed that biomolecules
like proteins are responsible for the reduction of metal ions and form encapsulating layer on the metal
ions (capping of PdNPs).
Results: Themorphological study showed an aggregated irregular shape using FEG-SEM. Elemental
composition and purity of the PdNPs were analyzed by EDS. HR-TEM revealed the size of the PdNPs
from 2 to 9 nm. Antibacterial study of the biosynthesized PdNPs was examined for gram-positive (Bacillus
subtilis and Stayphylococcus aureus) and gram-negative (Escherichia coli) bacteria using paper
strip method and PdNPs exhibited an excellent inhibition zone against Bacillus subtilis bacteria.
Significant antifungal study was observed against Candida albicans from yeast. In vitro anticancer
study of PdNPs which act as a drug carrier to kill the cancerous cells was performed on MCF-7 cell
line. Compared to the standard drug, PdNPs exhibited potent anticancer activity withIC50 value of