Background: Nano level chiral separation is necessary and demanding in the development
of the drug, genomic, proteomic, and other chemical and the environmental sciences. Few drugs exist
in human body cells for some days at nano level concentrations, that are out of the jurisdiction of the
detection by standard separation techniques. Likewise, the separation and identification of xenobiotics
and other environmental contaminants (at nano or low levels) are necessary for our healthiness.
Discussion: This review article reports the state-of-the-art of nano level chiral analyses by nano
liquid chromatography (NLC) and nano capillary electrophoresis (NCE). The optimization procedure
of the enantiomeric resolution is also deliberated. Also, the efforts are made to describe the chiral
resolution mechanisms of in NLC and NCE. Finally, the future perspectives are also emphasized.
Conclusion: This article will be beneficial for chiral chromatographers, academicians, pharmaceutical
industries, environmental researchers and Government regulation authorities.