Introduction: A new series of benzothiazole azo-ester derivatives was synthesized by
using Steglish esterification reaction.
Methods: All the synthesized compounds were screened for their anti-TB activities by in-vitro microplate
Alamar Blue assay method against M. tuberculosis (H37RV strain). All the compounds
showed activities and their MIC values were over the range of 1.6 μg/mL to 50 μg/mL. The compounds
4d and 4j showed superior activity with MIC 1.6 μg/mL compared to the standard drug
Streptomycin (MIC 6.25 μg/mL), Pyrazinamide (MIC 3.125 μg/mL) and Ciprofloxacin (MIC
3.125 μg/mL). Molecular docking study was carried out with enoyl acyl carrier reductase (InhA) of
M. tuberculosis and decaprenyl phosphoryl-D-ribose oxidase (DprE1).
Results: These studies showed that these compounds have more interaction with InhA protein
whereas some compounds could not be docked into DprE1.