Background: Biodiesel represents an alternative energy source with economic, social, environmental,
and technological advantages over nonrenewable fuels.
Objective: This study investigated two oils derived from plants of the Brazilian Amazon, andiroba
(Carapa sp.) oil and babassu (Orbignya sp.) oil, as possible triacylglyceride sources for the production
of biofuels by lipase B from Candida antarctica (CALB).
Methods: The production of biodiesel ethylic by enzymatic catalyst was carried out in 3-mL Erlenmeyer
flasks containing 150 mg (154 µL) of either babassu or andiroba oil, 475 µL of ethanol, and 15 mg
Results: The physical, chemical, and spectroscopic properties of these oils and their resulting biofuels
were also reported. CALB exhibited high activity in the alcoholysis of babassu and andiroba oils under
mild conditions and produced biodiesel ethyl esters in high yields (90% and 94%, respectively). The
oils and biodiesel were characterized using 1H NMR, Chromatography Gas–Mass Spectrometry (CGMS),
Fourier Transform Infrared Spectroscopy (FT-IR), and thermogravimetric analysis (TGA) being
the main findings of the study.
Conclusion: Ethanolysis of babassu and andiroba oils by catalysis enzymatic (CALB), showed to be a
successful route in the synthesis of ethylic biodiesel in high yield. The corresponding biodiesels were
characterized by 1H NMR spectra, GC-MS, FT-IR, and TGA analyses. Results described here provide
relevant information for the further research uses andiroba and babassu oils, the alternatives to other
oils in synthesis of biodiesel.