Acetate Kinase (AcK) is Essential for Microbial Growth, and Betel Compounds Potentially Target AcK, PhoP, and MDR Proteins in M. tuberculosis, V. cholerae, and Pathogenic E. coli: An in silico and in vitro Study

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Author(s): Sandeep Tiwari, Debmalya Barh*, M. Imchen, Eswar Rao, Ranjith K. Kumavath, S. Prabu Seenivasan, Arun Kumar Jaiswal, Syed Babar Jamal, Vanaja Kumar, Preetam Ghosh, Vasco Azevedo.

Journal Name: Current Topics in Medicinal Chemistry

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Mycobacterium tuberculosis, Vibrio cholerae, and pathogenic Escherichia coli are global concerns for public health. The emergence of multi-drug resistant (MDR) strains of these pathogens is creating additional challenges in controlling infections caused by these deadly bacteria. Recently, we reported that Acetate kinase (AcK) could be a broad-spectrum novel target in several bacteria including these pathogens. Here, using in silico and in vitro approaches we show that (i) AcK is an essential protein in pathogenic bacteria; (ii) natural compounds Chlorogenic acid and Pinoresinol from Piper betel and Piperidine derivative compound 6-oxopiperidine-3-carboxylic acid inhibit the growth of pathogenic E. coli and M. tuberculosis by targeting AcK with equal or higher efficacy than the currently used antibiotics; (iii) molecular modeling and docking studies show interactions between inhibitors and AcK that correlate with the experimental results; (iv) these compounds are highly effective even on MDR strains of these pathogens; (v) further, the compounds may also target bacterial two-component system proteins that help bacteria in expressing the genes related to drug resistance and virulence; and (vi) finally, all the tested compounds are predicted to have drug-like properties, suggesting that, these Piper betel derived compounds may be further tested for developing a novel class of broad-spectrum drugs against various common and MDR pathogens.

Keywords: Infectious disease, Multi-drug resistant, Natural compounds, Piper betel, Tuberculosis

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(E-pub Ahead of Print)
DOI: 10.2174/1568026619666190121105851
Price: $95

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