Background: The green synthesizing procedure of Silver nanoparticles (AgNPs) has
been performed through the usage of a natural polysaccharide; Arabic gum (AG) as a stabilizing/
capping agent. For characterization of AgNPs, TEM, particle size analyzer and UV-Vis were
Methods: The aim of our project was to identify biosynthesized AgNPs for sensing ammonia and
to explore its toxicity on Neuro-2A cells. We also reviewed the patents for biosynthesized AgNO3
and ammonia sensing. The optimal conditions for the synthesis of AgNPs in AG consist of utilizing
(0.1g) AG in dH2O (70 ml), 10 ml of 1 mM silver solution and 0.1 mM (AA) at 70°C stirring
for 30 minutes. The AgNPs cytotoxicity was evaluated on Neuro-2A cells; consequently, ammonia
was sensed with the lowest possible concentration of 10-6.
Results: Particle size analyzer displayed the mean diameter of about 70 nm for the sphericalshaped
Ag-NPs. UV–Vis revealed that the prepared AgNPs were ammonia sensitive in solution as
the concentration of ammonia was increased. The cytotoxicity of AgNPs indicated lower Cell viability
at higher concentrations of the AG-capped AgNPs.
Conclusion: By synthesis of AgNPs in GA by using AA, we successfully prepared a sensor to diagnose
ammonia in a cell and sensing its level at concentrations of 10-6 M. In this study, no therapeutic
application has been shown, but this method could be utilized industrially for therapeutic
purposes in the future.