Differential Expression of Seven De-sumoylation Enzymes (SENPs) in Major Ocular Tissues of Mouse Eye

Author(s): Jia-Wen Xiang, Lan Zhang, Xiangcheng Tang, Yuan Xiao, Yunfei Liu, Ling Wang, Fangyuan Liu, Xiao-Dong Gong, Jia-Ling Fu, Lan Yang, Zhongwen Luo, David Wan-Cheng Li*.

Journal Name: Current Molecular Medicine

Volume 18 , Issue 8 , 2018

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Abstract:

Purpose: Protein Sumoylation is one of the most important and prevalent posttranscriptional modification. Increasing evidence have shown that the SENPs (sentrin/SUMOspecific proteases) are critical for steady-state levels of SUMO modification of target proteins, and protein de-sumoylation modulates a great diversity of biological processes including transcription, development, differentiation, neuroprotection, as well as pathogenesis. In the vertebrate eye, we and others have previously shown that sumoylation participated in the differentiation of major ocular tissues including retina and lens. However, the biological significance of seven SENP enzymes: SENP1 to 3 and SENP5 to 8 have not be fully investigated in the ocular tissues.

Methods: The 5 major ocular cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing fetal bovine serum (FBS) or rabbit serum (RBS) and 1% Penicillin- Streptomycin. The mRNA levels were analysed with qRT-PCR. The protein levels were determined with western blot analysis and quantitated with Image J.

Results: At the mRNA level, all SENPs were highly expressed in retina, and much reduced expression patterns in cornea, lens epithelium and lens fiber. At the protein level, SENP1 to -3, and SENP6 were highly abundant in cornea, while SENP5, SENP7 and SENP8 were enriched in retina, and these SENPs were relatively less abundant in lens tissues.

Conclusion: Our results for the first time established the differentiation expression patterns of the 7 de-sumoylation enzymes (SENPs), which provides a basis for further investigation of protein desumoylation functions in vertebrate eye.

Keywords: Sumoylation, De-sumoylation, SENP, retina, lens, cornea.

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Article Details

VOLUME: 18
ISSUE: 8
Year: 2018
Page: [533 - 541]
Pages: 9
DOI: 10.2174/1566524019666190112132103
Price: $58

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