Bioreactors Design, Types, Influencing Factors and Potential Application in Dentistry. A Literature Review

Author(s): Neeraj Malhotra*.

Journal Name: Current Stem Cell Research & Therapy

Volume 14 , Issue 4 , 2019

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Abstract:

Objectives: A variety of bioreactors and related approaches have been applied to dental tissues as their use has become more essential in the field of regenerative dentistry and dental tissue engineering. The review discusses the various types of bioreactors and their potential application in dentistry.

Methods: Review of the literature was conducted using keywords (and MeSH) like Bioreactor, Regenerative Dentistry, Fourth Factor, Stem Cells, etc., from the journals published in English. All the searched abstracts, published in indexed journals were read and reviewed to further refine the list of included articles. Based on the relevance of abstracts pertaining to the manuscript, full-text articles were assessed.

Results: Bioreactors provide a prerequisite platform to create, test, and validate the biomaterials and techniques proposed for dental tissue regeneration. Flow perfusion, rotational, spinner-flask, strain and customize-combined bioreactors have been applied for the regeneration of bone, periodontal ligament, gingiva, cementum, oral mucosa, temporomandibular joint and vascular tissues. Customized bioreactors can support cellular/biofilm growth as well as apply cyclic loading. Center of disease control & dip-flow biofilm-reactors and micro-bioreactor have been used to evaluate the biological properties of dental biomaterials, their performance assessment and interaction with biofilms. Few case reports have also applied the concept of in vivo bioreactor for the repair of musculoskeletal defects and used customdesigned bioreactor (Aastrom) to repair the defects of cleft-palate.

Conclusions: Bioreactors provide a sterile simulated environment to support cellular differentiation for oro-dental regenerative applications. Also, bioreactors like, customized bioreactors for cyclic loading, biofilm reactors (CDC & drip-flow), and micro-bioreactor, can assess biological responses of dental biomaterials by simultaneously supporting cellular or biofilm growth and application of cyclic stresses.

Keywords: Biomaterials, bioreactor, dentistry, fourth element, micro-bioreactors, scaffold, stem cells.

[1]
Depprich R, Handschel J, Wiesmann HP, Jäsche-Meyer J, Meyer U. Use of bioreactors in maxillofacial tissue engineering. Br J Oral Maxillofac Surg 2008; 46(5): 349-54.
[2]
Plunkett N, O’Brien FJ. Bioreactors in tissue engineering. Technol Health Care 2011; 19(1): 55-69.
[3]
Darling EM, Athanasiou KA. Biomechanical strategies for articular cartilage regeneration. Ann Biomed Eng 2003; 31(9): 1114-24.
[4]
Malhotra N, Kundabala M, Acharya S. Current strategies and applications of tissue engineering in dentistry-a review. Part 1. Dent Update 2009; 36(9): 577-82.
[5]
Malhotra N, Mala K. Regenerative endodontics as a tissue engineering approach. Past, current and future – a review. Australian Endodontic J 2012; 38(3): 137-48.
[6]
Ingber DE, Dike L, Hansen L, et al. Cellular tensegrity: Exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis. Int Rev Cytol 1994; 150: 173-224.
[7]
Liu M, Liu N, Zang R, Li Y, Yang ST. Engineering stem cell niches in bioreactors. World J Stem Cells 2013; 5(4): 124-35.
[8]
Korossis SA, Bolland F, Kearney JN, Fisher J, Ingham E. Bioreactors in Tissue Engineering.In: Ashammakhi N, Reis R L, editors. Topics in Tissue Engineering [e-book]. Volume 2. U.K. 2005. Available from: Biomaterials and Tissue Engineering Group.
[9]
Bono E, Mathes SH, Franscini N, Graf-Hausner U. Tissue engineering-the gateway to regenerative medicine. CHIMIA 2010; 64(11): 808-12.
[10]
Cartmell SH, Porter BD, Garcia AJ, Guldberg RE. Effects of medium perfusion rate on cell seeded three dimensional bone constructs in vitro. Tissue Eng 2003; 9: 1197-203.
[11]
Goldstein AS, Juarez TM, Helmke CD, Gustin MC, Mikos AG. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials 2001; 22(11): 1279-88.
[12]
Cimetta E, Vunjak-Novakovic G. Microscale technologies for regulating human stem cell differentiation. Exp Biol Med 2014; 239(9): 1255-63.
[13]
Partap S, Plunkett NA, O’Brien FJ. Bioreactors in Tissue Engineering, Tissue Engineering, Daniel Eberli (Ed.), InTech, 2010. DOI: 10.5772/8579. Available from:. https://www.intechopen.com/books/tissue-engineering/bioreactors-in-tissue-engineering
[14]
Langer R, Vacanti JP. Tissue engineering. Science 1993; 260(5110): 920-6.
[15]
Martin Y, Vermette P. Bioreactors for tissue mass culture: Design, characterization, and recent advances. Biomaterials 2005; 26(35): 7481-503.
[16]
Mygind T, Stiehler M, Baatrup A, et al. Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds. Biomaterials 2007; 28(6): 1036-47.
[17]
Schwarz RP, Goodwin TJ, Wolf DA. Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity. J Tissue Cult Methods 1992; 14(2): 51-7.
[18]
Lanza P, Langer R, Vacanti J. Tissue culture bioreactors In ‘Principles of Tissue Engineering’ 2nd Edition, 1999; 151-65
[19]
Yu X, Botchwey EA, Levine EM, Pollack SR, Laurencin CT. Bioreactor-based bone tissue engineering: The influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization. Proc Natl Acad Sci USA 2004; 101(31): 11203-8.
[20]
Meyer U, Büchter A, Nazer N, Wiesmann HP. Design and performance of a bioreactor system for mechanically promoted three-dimensional tissue engineering. Br J Oral Maxillofac Surg 2006; 44(2): 134-40.
[21]
Jaasma MJ, Plunkett NA, O’Brien FJ. Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds. J Biotechnol 2008; 133(4): 490-6.
[22]
Huang CY, Hagar KL, Frost LE, Sun Y, Cheung HS. Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells. Stem Cells 2004; 22(3): 313-23.
[23]
McMahon LA, Reid AJ, Campbell VA, Prendergast PJ. Regulatory effects of mechanical strain on the chondrogenic differentiation of MSCs in a collagen-GAG scaffold: Experimental and computational analysis. Ann Biomed Eng 2008; 36(2): 185-94.
[24]
Garvin J, Qi J, Maloney M, Banes AJ. Novel system for engineering bioartificial tendons and application of mechanical load. Tissue Eng 2003; 9(5): 967-79.
[25]
Darling EM, Athanasiou KA. Articular cartilage bioreactors and bioprocesses. Tissue Eng 2003; 9(1): 9-26.
[26]
Watanabe S, Inagaki S, Kinouchi I, Takai H, Masuda Y, Mizuno S. Hydrostatic pressure/perfusion culture system designed and validated for engineering tissue. J Biosci Bioeng 2005; 100(1): 105-11.
[27]
Niklason LE, Langer RS. Advances in tissue engineering of blood vessels and other tissues. Transpl Immunol 1997; 5(4): 303-6.
[28]
Hoerstrup SP, Sodian R, Sperling JS, Vacanti JP, Mayer JE Jr. New pulsatile bioreactor for in vitro formation of tissue engineered heart valves. Tissue Eng 2000; 6(1): 75-9.
[29]
Kasper FK, Melville J, Shum J, Wong M, Young S. Tissue engineered prevascularized bone and soft tissue flaps. Oral Maxillofac Surg Clin North Am 2017; 29(1): 63-73.
[30]
Tatara AM, Wong ME, Mikos AG. In vivo bioreactors for mandibular reconstruction. J Dent Res 2014; 93(12): 1196-202.
[31]
Khouri RK, Koudsi B, Reddi H. Tissue transformation into bone in vivo. A potential practical application. JAMA 1991; 266(14): 1953-5.
[32]
Thomson RC, Mikos AG, Beahm E, et al. Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds. Biomaterials 1999; 20(21): 2007-18.
[33]
Miller MJ, Goldberg DP, Yasko AW, et al. Guided bone growth in sheep: a model for tissue engineered bone flaps. Tissue Eng 1996; 2(1): 51-9.
[34]
Cheng M-H, Brey EM, Allori AC, et al. Ovine model for engineering bone segments. Tissue Eng 2005; 11(1-2): 214-25.
[35]
Cheng M-H, Brey EM, Allori AC, et al. Periosteum guided prefabrication of vascularized bone of clinical shape and volume. Plast Reconstr Surg 2009; 124(3): 787-95.
[36]
Brey EM, Cheng M-H, Allori A, et al. Comparison of guided bone formation from periosteum and muscle fascia. Plast Reconstr Surg 2007; 119(4): 1216-22.
[37]
Geuze RE, Theyse LFH, Kempen DHR, et al. A differential effect of bone morphogenetic protein-2 and vascular endothelial growth factor release timing on osteogenesis at ectopic and orthotopic sites in a large-animal model. Tissue Eng Part A 2012; 18(19-20): 2052-62.
[38]
Gitelis S, Wilkins RM, Yasko AW. BMP’s and cancer: Is the risk real? AAOS Now 2008; 2: 31.
[39]
Warnke PH, Springer IN, Acil Y, et al. The mechanical integrity of in vivo engineered heterotopic bone. Biomaterials 2006; 27(7): 1081-7.
[40]
Tatara AM, Kretlow JD, Spicer PP, et al. Autologously generated tissue-engineered bone flaps for reconstruction of large mandibular defects in an ovine model. Tissue Eng Part A 2015; 21(9-10): 1520-8.
[41]
Huang RL, Kobayashi E, Liu K, Li O. Bone graft prefabrication following the in vivo bioreactor principle. EBioMedicine 2016; 12: 43-54.
[42]
Cimetta E, Sirabella D, Yeager K, et al. Microfluidic bioreactor for dynamic regulation of early mesodermal commitment in human pluripotent stem cells. Lab Chip 2013; 13(3): 355-64.
[43]
Khademhosseini A, Langer R, Borenstein J, Vacanti JP. Microscale technologies for tissue engineering and biology. Proc Natl Acad Sci USA 2006; 103(8): 2480-7.
[44]
Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol 2011; 21(12): 745-54.
[45]
Cimetta E, Figallo E, Cannizzaro C, Elvassore N, Vunjak-Novakovic G. Micro-bioreactor arrays for controlling cellular environments: design principles for human embryonic stem cell applications. Methods 2009; 47(2): 81-9.
[46]
Jeon NL, Dertinger SKW, Chiu DT, et al. Generation of solution and surface gradients using microfluidic systems. Langmuir 2000; 16(22): 8311-6.
[47]
Cimetta E, Figallo E, Cannizzaro C, Elvassore N, Vunjak-Novakovic G. Micro-bioreactor arrays for controlling cellular environments: design principles for human embryonic stem cell applications. Methods 2009; 47(2): 81-9.
[48]
Kane RS, Takayama S, Ostuni E, Ingber DE, Whitesides GM. Patterning proteins and cells using soft lithography. Biomaterials 1999; 20(23-24): 2363-76.
[49]
Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg Am 2004; 86-A(7): 1541-58.
[50]
Rubin CT, Lanyon LE. Osteoregulatory nature of mechanical stimuli: Function as a determinant for adaptive remodeling in bone. J Orthop Res 1987; 5(2): 300-10.
[51]
Mohyeldin A, Garzón-Muvdi T, Quiñones-Hinojosa A. Oxygen in stem cell biology: A critical component of the stem cell niche. Cell Stem Cell 2010; 7(2): 150-61.
[52]
Malda J, Martens DE, Tramper J, van Blitterswijk CA, Riesle J. Cartilage tissue engineering: Controversy in the effect of oxygen. Crit Rev Biotechnol 2003; 23(3): 175-94.
[53]
Lovett M, Rockwood D, Baryshyan A, Kaplan DL. Simple modular bioreactors for tissue engineering: A system for characterization of oxygen gradients, human mesenchymal stem cell differentiation, and prevascularization. Tissue Eng Part C Methods 2010; 16(6): 1565-73.
[54]
Ivanović Z, Dello Sbarba P, Trimoreau F, et al. Primitive human HPCs are better maintained and expanded in vitro at 1 percent oxygen than at 20 percent. Transfusion 2000; 40(12): 1482-8.
[55]
Yoshida Y, Takahashi K, Okita K, Ichisaka T, Yamanaka S. Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 2009; 5(3): 237-41.
[56]
Patwari P, Lee RT. Mechanical control of tissue morphogenesis. Circ Res 2008; 103(3): 234-43.
[57]
Hung CT, Mauck RL, Wang CC, Lima EG, Ateshian GA. A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng 2004; 32(1): 35-49.
[58]
Chen KD, Li YS, Kim M, et al. Mechanotransduction in response to shear stress. Roles of receptor tyrosine kinases, integrins, and Shc. J Biol Chem 1999; 274(26): 18393-400.
[59]
Ignatius A, Blessing H, Liedert A, et al. Tissue engineering of bone: Effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials 2005; 26(3): 311-8.
[60]
Discher DE, Janmey P, Wang YL. Tissue cells feel and respond to the stiffness of their substrate. Science 2005; 310(5751): 1139-43.
[61]
McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 2004; 6(4): 483-95.
[62]
Jones D, Leivseth G, Tenbosch J. Mechano-reception in osteoblast-like cells. Biochem Cell Biol 1995; 73(7-8): 525-34.
[63]
Meyer U, Joos U, Wiesmann HP. Biological and biophysical principles in extracorporal bone tissue engineering. Part III. Int J Oral Maxillofac Surg 2004; 33(7): 635-41.
[64]
Masi L, Franchi A, Santucci M, et al. Adhesion, growth, and matrix production by osteoblasts on collagen substrata. Calcif Tissue Int 1992; 51(3): 202-12.
[65]
Krishnan L, Weiss JA, Wessman MD, Hoying JB. Design and application of a test system for viscoelastic characterization of collagen gels. Tissue Eng 2004; 10(1-2): 241-52.
[66]
Buschmann MD, Gluzband YA, Grodzinsky AJ, Hunziker EB. Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture. J Cell Sci 1995; 108(4): 1497-508.
[67]
Martin I, Obradovic B, Treppo S, et al. Modulation of the mechanical properties of tissue engineered cartilage. Biorheology 2000; 37(1-2): 141-7.
[68]
Finlay S, Seedhom BB, Carey DO, et al. In vitro engineering of high modulus cartilage-like constructs. Tissue Eng Part C Methods 2016; 22(4): 382-97.
[69]
Sargent CY, Berguig GY, Kinney MA, et al. Hydrodynamic modulation of embryonic stem cell differentiation by rotary orbital suspension culture. Biotechnol Bioeng 2010; 105(3): 611-26.
[70]
Pavalko FM, Chen NX, Turner CH, et al. Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions. Am J Physiol 1998; 275(1): C1591-601.
[71]
Botchwey E A, Pollack S R, Levine E M, Laurencin C T. Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system. J Biomed Mater Res 200; 55(2): 242-53
[72]
Sikavitsas VI, Bancroft GN, Mikos AG. Formation of three-dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J Biomed Mater Res 2002; 62(1): 136-48.
[73]
Pei M, Solchaga LA, Seidel J, et al. Bioreactors mediate the effectiveness of tissue engineering scaffolds. FASEB J 2002; 16(12): 1691-4.
[74]
Hartig M, Joos U, Wiesmann HP. Capacitively coupled electric fields accelerate proliferation of osteoblast-like primary cells and increase bone extracellular matrix formation in vitro. Eur Biophys J 2000; 29(7): 499-506.
[75]
Wiesmann H, Hartig M, Stratmann U, Meyer U, Joos U. Electrical stimulation influences mineral formation of osteoblast-like cells in vitro. Biochim Biophys Acta 2001; 1538(1): 28-37.
[76]
Yamada M, Tanemura K, Okada S, et al. Electrical stimulation modulates fate determination of differentiating embryonic stem cells. Stem Cells 2007; 25(3): 562-70.
[77]
Villa-Diaz LG, Ross AM, Lahann J, Krebsbach PH. The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings. Stem Cells 2013; 31(1): 1-7.
[78]
Steiner D, Khaner H, Cohen M, et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 2010; 28(4): 361-4.
[79]
Olmer R, Haase A, Merkert S, et al. Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium. Stem Cell Res 2010; 5(1): 51-64.
[80]
Amit M, Chebath J, Margulets V. Suspension culture of undifferentiated human embryonic and induced pluripotent stem cells. Stem Cell Rev 2010; 6(2): 248-59.
[81]
Singh H, Mok P, Balakrishnan T, et al. Up-scaling single cell-inoculated suspension culture of human embryonic stem cells. Stem Cell Res 2010; 4(3): 165-79.
[82]
Mathes SH, Wohlwend L, Uebersax L, et al. A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnol Bioeng 2010; 107(6): 1029-39.
[83]
Cheung JW, McCulloch CA, Santerre JP. Establishing a gingival fibroblast phenotype in a perfused degradable polyurethane scaffold: mediation by TGF-β1, FGF-2, β1-integrin, and focal adhesion kinase. Biomaterials 2014; 35(38): 10025-32.
[84]
Gault P, Black A, Romette JL, et al. Tissue-engineered ligament: Implant constructs for tooth replacement. J Clin Periodontol 2010 1; 37(8): 750-8
[85]
Jin QM, Zhao M, Webb SA, et al. Cementum engineering with three-dimensional polymer scaffolds. J Biomed Mater Res A 2003; 67(1): 54-60.
[86]
El-Backly RM, Massoud AG, El-Badry AM, et al. Regeneration of dentine/pulp-like tissue using a dental pulp stem cell/poly(lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits. Aust Endod J 2008; 34(2): 52-67.
[87]
Gonçalves SB, Dong Z, Bramante CM, et al. Tooth slice-based models for the study of human dental pulp angiogenesis. J Endod 2007; 33(7): 811-4.
[88]
Demarco FF, Casagrande L, Zhang Z, et al. Effects of morphogen and scaffold porogen on the differentiation of dental pulp stem cells. J Endod 2010; 36(11): 1805-11.
[89]
Sharifpoor S, Simmons CA, Labow RS, Paul Santerre J. Functional characterization of human coronary artery smooth muscle cells under cyclic mechanical strain in a degradable polyurethane scaffold. Biomaterials 2011; 32(21): 4816-29.
[90]
Niklason LE, Gao J, Abbott WM, et al. Functional arteries grown in vitro. Science 1999; 284(5413): 489-93.
[91]
Bilodeau K, Couet F, Boccafoschi F, Mantovani D. Design of a perfusion bioreactor specific to the regeneration of vascular tissues under mechanical stresses. Artif Organs 2005; 29(11): 906-12.
[92]
Sodian R, Lemke T, Fritsche C, et al. Tissue-engineering bioreactors: A new combined cell-seeding and perfusion system for vascular tissue engineering. Tissue Eng 2002; 8(5): 863-70.
[93]
Ruiz JP, Ecker N, Pawley D, Cheung H. Recent advances in bioreactors in tissue engineering and regenerative medicine. Curr Tissue Eng 2013; 2(2): 133-44.
[94]
Konopnicki S, Sharaf B, Resnick C, et al. Tissue-engineered bone with 3-dimensionally printed β-tricalcium phosphate and polycaprolactone scaffolds and early implantation: An in vivo pilot study in a porcine mandible model. J Oral Maxillofac Surg 2015; 73(5): 1016.e1-1016.e11.
[95]
Lee JM, Kim MG, Byun JH, et al. The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells. Maxillofac Plast Reconstr Surg 2017; 39(1): 7.
[96]
Terheyden H, Jepsen S, Rueger DR. Mandibular reconstruction in miniature pigs with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: A preliminary study. Int J Oral Maxillofac Surg 1999; 28(6): 461-3.
[97]
Zhou M, Peng X, Mao C, et al. Primate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situ. Biomaterials 2010; 31(18): 4935-43.
[98]
Kokemueller H, Spalthoff S, Nolff M, et al. Prefabrication of vascularized bioartificial bone grafts in vivo for segmental mandibular reconstruction: experimental pilot study in sheep and first clinical application. Int J Oral Maxillofac Surg 2010; 39(4): 379-87.
[99]
Heliotis M, Lavery KM, Ripamonti U, Tsiridis E, di Silvio L. Transformation of a prefabricated hydroxyapatite/osteogenic protein-1 implant into a vascularised pedicled bone flap in the human chest. Int J Oral Maxillofac Surg 2006; 35(3): 265-9.
[100]
Cheng MH, Brey EM, Ulusal BG, Wei FC. Mandible augmentation for osseointegrated implants using tissue engineering strategies. Plast Reconstr Surg 2006; 118(1): 1e-4e.
[101]
Warnke PH, Springer IN, Wiltfang J, et al. Growth and transplantation of a custom vascularised bone graft in a man. Lancet 2004; 364(9436): 766-70.
[102]
Orringer JS, Shaw WW, Borud LJ, et al. Total mandibular and lower lip reconstruction with a prefabricated osteocutaneous free flap. Plast Reconstr Surg 1999; 104(3): 793-7.
[103]
Mesimäki K, Lindroos B, Törnwall J, et al. Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells. Int J Oral Maxillofac Surg 2009; 38(3): 201-9.
[104]
Alhadlaq A, Mao JJ. Tissue-engineered neogenesis of human-shaped mandibular condyle from rat mesenchymal stem cells. J Dent Res 2003; 82(12): 951-6.
[105]
Grayson WL, Fröhlich M, Yeager K, et al. Engineering anatomically shaped human bone grafts. Proc Natl Acad Sci USA 2010; 107(8): 3299-304.
[106]
Almela T, Brook IM, Moharamzadeh K. Development of three-dimensional tissue engineered bone-oral mucosal composite models. J Mater Sci Mater Med 2016; 27(4): 65.
[107]
Paganelli C, Fontana P, Porta F, et al. Indications on suitable scaffold as carrier of stem cells in the alveoloplasty of cleft palate. J Oral Rehabil 2006; 33(8): 625-9.
[108]
Khvostenko D, Salehi S, Naleway SE. Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps. Dent Mater 2015; 31(6): 702-10.
[109]
Khvostenko D, Hilton TJ, Ferracane JL, Mitchell JC, Kruzic JJ. Bioactive glass fillers reduce bacterial penetration into marginal gaps for composite restorations. Dent Mater 2016; 32(1): 73-81.
[110]
Rudney JD, Chen R, Lenton P, et al. A reproducible oral microcosm biofilm model for testing dental materials. J Appl Microbiol 2012; 113(6): 1540-53.
[111]
Li Y, Carrera C, Chen R, et al. Degradation in the dentin-composite interface subjected to multi-species biofilm challenges. Acta Biomater 2014; 10(1): 375-83.
[112]
Chen X, Hirt H, Li Y, Gorr SU, Aparicio C. Antimicrobial GL13K peptide coatings killed and ruptured the wall of Streptococcus gordonii and prevented formation and growth of biofilms. PLoS One 2014; 9(11): e111579.
[113]
Bächle M, Mahi MA, Kohal RJ. On-line analysis of CAL72 cells on two different titanium surfaces in a perfusion micro-bioreactor. Dent Mater 2005; 21(7): 633-40.


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VOLUME: 14
ISSUE: 4
Year: 2019
Page: [351 - 366]
Pages: 16
DOI: 10.2174/1574888X14666190111105504
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