Background: CYP2C19 is an important genetic factor modulating clopidogrel dose
Objective: Therefore, a simple and economic genotyping method for predicting the clopidogrel
dose of patients would be useful in clinical applications.
Method: In this study, the MGB blocker ARMS real-time PCR contained two forward primers,
two MGB blockers and a common reverse primer have been used for CYP2C19*2, *3 and *17
Results: Results showed that heterozygotes and homozygotes of CYP2C19*2, *3 and *17 could be
distinguished by the MGB blocker ARMS real-time PCR successfully. In the Chinese population,
patients with allele frequencies of CYP2C19*2, *3, and *17 are 18.43%, 3.03% and 0.76%,
Conclusion: This study indicates that the MGB blocker ARMS real-time PCR will be a simple,
economical method for the rapid detection of SNPs in CYP2C19.