MGB Block ARMS Real-Time PCR for Diagnosis of CYP2C19 Mutation in a Chinese Population

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Author(s): Xiwen Jiang*, Yue Liu, Taosheng Huang, Xiaoya Zhu, Xiaoxie Xu.

Journal Name: Current Bioinformatics

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Background: CYP2C19 is an important genetic factor modulating clopidogrel dose requirement. Objective: Therefore, a simple and economic genotyping method for predicting the clopidogrel dose of patients would be useful in clinical applications. Method: In this study, the MGB blocker ARMS real-time PCR contained two forward primers, two MGB blockers and a common reverse primer have been used for CYP2C19*2, *3 and *17 substitutions. Results: Results showed that heterozygotes and homozygotes of CYP2C19*2, *3 and *17 could be distinguished by the MGB blocker ARMS real-time PCR successfully. In the Chinese population, patients with allele frequencies of CYP2C19*2, *3, and *17 are 18.43%, 3.03% and 0.76%, respectively. Conclusion: This study indicates that the MGB blocker ARMS real-time PCR will be a simple, economical method for the rapid detection of SNPs in CYP2C19.

Keywords: MGB, Blocker, ARMS, CYP2C19

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(E-pub Ahead of Print)
DOI: 10.2174/1574893614666190109154252