Background: Chiral separation involves many phenomena in which the elution order of
the enantiomers has its unique position. The phenomenon of elution order of the enantiomers has also
been used in the determination of optical purity which is favorable to elute the major component after
minor enantiomeric impurity but the main problem is that, this phenomenon is rare.
Results: This review rumors the reversal order of elution of many chiral molecules in HPLC. Besides,
this review pronounces the effects of pH, derivatisation of drugs, the composition of the mobile
phase, and temperature on the reversal order of elution of chiral drugs. The efforts are also made
to discuss the possible future perspectives of reversal order of elution.
Conclusion: Various parameters such as pH, mobile phase composition, temperature, and chemical
structure of the analytes play a role in the phenomena of the reversal order of elution of many chiral
molecules which are discussed in the article.