Background: The lack of treatment options for patients with chemotherapy-resistant cancers
is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of
heterocyclic compounds that exhibit a broad spectrum of antitumor activities.
Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives
by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side
Method: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and
their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining.
Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and
Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer
cell line MDA-MB-231 in vitro.
Keywords: 5-tetrazine derivatives, in vitro experiment, MDA-MB-231 cell line, breast cancer, 3, 6-dichloro-1, 5-tetrazine, apoptosis
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