Lab-on-a-chip Dielectrophoretic Manipulation of Beta-2 Microglobulin for Toxin Removal in An Artificial Kidney

(E-pub Ahead of Print)

Author(s): Muhammad Izzuddin Abd Samad, Aminuddin Ahmad Kayani, Ahmad Sabirin Zoolfakar, Azrul Azlan Hamzah, Burhanuddin Yeop Majlis, Muhamad Ramdzan Buyong*.

Journal Name: Micro and Nanosystems

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Abstract:

This paper presents a fundamental study of protein manipulation under the influence of dielectrophoretic (DEP) force for a lab-on-a-chip platform Protein manipulation dependent on the polarisation factor of protein when exposed to an electric field. A microfluidic device and measurement system are used to characterise the human beta-2 microglobulin (β2M) protein via lateral attractive forces and vertical repulsive forces by means of DEP responses. The manipulation of the β2M protein was conducted using a microfluidic platform with a tapered DEP microelectrode and the protein concentration was quantified based on a biochemical interaction using an enzyme-linked immunosorbent assay (ELISA). The protein distribution has been analysed based on the β2M concentration for each microfluidic outlet. At 300 kHz, the protein experienced a negative DEP (nDEP) with 83.3 % protein distribution on the middle microchannel. In contrast, the protein experienced a positive DEP (pDEP) at 1.2 MHz with 78.7% of protein on the left and right sides of the microchannel. This concept proved that the tapered DEP microelectrode is capable of manipulating a β2M via particle polarisation, hence making it suitable to be utilised for purifying proteins in biomedical application.

Keywords: Dielectrophoresis, Beta-2-Microglobulin, Lab on Chip , Artificial Kidney

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1876402911666181218145459
Price: $95