Introduction: In cancer therapeutics, several new classes of small molecules based targeted
drug options are reported including peptide mimetic and small RNAs therapeutics. Small RNAs
represent a class of short non-coding endogenous RNAs that play an important role in transcriptional
and post transcriptional gene regulation among varied types of species including plants and animals.
Methods: To address the role of small RNAs from plant sources upon cancer cells, authors report on
the effects of small RNAs fraction of potato in in-vitro model of human derived HeLa cancer cells.
This paper reports the anti-proliferative and anti-survival effect of small RNAs fraction of
S. tuberosum L. (potato) tuber tissue. Here, authors employed small RNAs fractionation protocol,
cell viability, cell cytotoxicity MTT, PI stained cell cycle analysis and FITC-Annexin-V/PI stained
Results: In this paper, small RNAs fractions of potato clearly indicate 40-50% inhibition of HeLa
cell proliferation and viability. Interestingly, flow cytometer data point out appreciable increase from
7% to 14% of S-phase in HeLa cells by displaying the presence of an S-phase cell cycle arrest. Further,
arrest in S-phase of HeLa cells is also supported by an appreciable increase in total <2N plus
>4N DNA containing HeLa cells over 2N containing HeLa cells. For apoptotic assay, data suggest a
significant increase in apoptotic HeLa cells from (5%) control treated HeLa cells to (18%) small
RNAs treated HeLa cells.
Conclusion: Taken together, findings suggest that small RNAs fractions of potato can induce Sphase
cell cycle arrest and these agents can act as an anti-proliferative agent in HeLa cells. This paper
proposes a huge scope for novel finding to dissect out the small RNAs target within HeLa cells
and other cancer cell types.