Madhuca indica Inhibits Breast Cancer Cell Proliferation by Modulating COX-2 Expression

Author(s): Paramita Ghosh, Debarpan Mitra, Sreyashi Mitra, Sudipta Ray, Samir Banerjee, Nabendu Murmu*.

Journal Name: Current Molecular Medicine

Volume 18 , Issue 7 , 2018

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Abstract:

Background: Madhuca indica belongs to the family sapotaceae, commonly known as Mahua. It is primarily known for alcoholic beverage production and is reported to have anti-inflammatory, analgesic and antipyretic properties. Madhuca indica has also been reported to be effective in several diseases.

Objective: This study was undertaken to check the anticancer efficacy and chemopreventive effect of methanolic extract of Mahua flower (ME) on human breast cancer cell lines MCF-7 and MDA-MB-468.

Method: The cytotoxic and anti-proliferative effects on MCF-7 and MDA-MB-468 cells were studied by MTT, hexosaminidase and colony formation assay. Expression of caspase 3/7 was assessed by flow cytometry and western blot analysis. Expression of COX-2 was evaluated by western blot analysis, luciferase assay and mRNA analysis.

Results: ME inhibited the proliferation of breast cancer cells by inducing apoptosis through up-regulating the expression of Caspase 3/7 (P < 0.0001). Our results showed a decrease in the expression of COX-2 mRNA and COX-2 protein in both MCF-7 and MDA-MB-468 cells with an increase in ME concentration. Furthermore synergistic effect of ME and chemotherapeutic drug paclitaxel was also studied in MCF-7 and MDA-MB- 468 cells which were found to be more effective (P < 0.0001) than treatment of either ME or paclitaxel alone. Results were analyzed by ANOVA and Pearson correlation analysis.

Conclusion: All these experiments suggest that ME inhibits breast cancer cell proliferation and apoptosis by inhibiting the expression of COX-2 in MCF-7 and MDAMB- 468 cells. This work further highlighted that ME may enhance the potentiality of paclitaxel in breast cancer treatment.

Keywords: MCF-7, MDA-MB-468, ME, COX-2, paclitaxel, AKT, NF-κB.

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Article Details

VOLUME: 18
ISSUE: 7
Year: 2018
Page: [459 - 474]
Pages: 16
DOI: 10.2174/1566524019666181212100808
Price: $58

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