Aim: The microRNAs regulate the expression of multiple genes involved in diseases such
as cancer, diabetes and cardiovascular disease. In this study, we have investigated the association between
the miR-224 gene polymorphism (rs188519172A>G) and susceptibility of coronary artery disease
Methodology: Hundred CAD patients and 100-matched healthy control were included. Genotyping
of the miR-224 (rs188519172A>G) polymorphism was performed using Amplification refractory
mutation system PCR method (ARMS-PCR).
Result: A significant difference was observed in the genotype distribution among CAD patients and
healthy controls (P=0.018). The frequencies of all three genotypes GG, GA, AA reported in the patient’s
samples were 33%, 66% and 01%, and in the healthy controls samples, were 16%, 82% and
2% respectively. A multivariate analysis based on logistic regression was conducted for each group
to estimate the association between miR-224 rs188519172 genotypes and risk to coronary artery disease.
Results show that the miR-224 (rs188519172 A>G) polymorphism was associated with a decreased
risk to CAD in a codominant model, GA genotype vs. GG (OR = 0.39 (95 % CI, 0.19-0.76),
RR 0.58 (0.38-0.90, P=0.006). In the dominant model, (GA+AA vs. GG), there was also a significant
association with the OR=0.38 (95 % CI (0.19-0.76), RR 0.58 (0.38-0.89), and P=0.006. Whereas, in
the recessive model, (GG+GA vs. AA), there was no significant association of CAD with OR=0.49
(95% CI (0.044-5.54), RR 0.74 (0.33-1.68), and P=0.48.
Conclusion: Our findings indicated that miR-224 (rs188519172) GA genotype is associated with a
decreased susceptibility to CAD.