Aim and Background: The rationale of this study is that, treatment of asthmatic Guinea pig
with combined administration of Montelukast sodium and Green Tea Extract (GTE) as a single capsule
will mitigate the inflammatory injury in the airways and weaken the asthmatic response. Recent patents
for the treatment of asthma researched a polyphenolic alternatives for antiasthmatic combination therapy,
especially for those patients who remains unresponsive or poorly responsive for current asthma
therapy (US7232585B2). Synergistic activity of green tea polyphenols and therapeutic, prophylatic
agents are also reported in some recent patents (US20120172423A1, US20150320696A1). The present
work is therefore aimed, to study the effect of Montelukast Sodium capsules coformulated with GTE on
oxidative stress markers including Malondialdehyde (MDA), Glutathione (GSH) in different organs
and Oxygen Radical Absorbance Capacity (ORAC) assay in plasma.
Materials and Methods: Guinea pigs were placed in histamine chamber and exposed to an aerosol
challenge of 0.2% w/v histamine dihydrochloride in distilled water using pressurized air driven nebulizer
at a pressure of 0.05 MPa-0.106 MPa for one week. After that, they were divided in to four groups
of three each; control, asthmatic control, asthmatic treated with marketed preparation and asthmatic
received developed capsules. After oral administration of formulations for three days, pigs were scarificed
and oxidative stress markers level including cytoarchitectural manifestation in tissues was studied.
Results: In comparison with the healthy control group, MDA level of the asthmatic animal liver and
lung was found to be elevated as 0.059 ± 0.031(p < 0.002) and 0.802 ± 0.310 (p < 0.005) respectively,
whereas GSH level was declined as 13.223 ± 1.485 (p < 0.0001) in liver and 3.037 ± 0.282 (p < 0.0004)
in lung tissues. TAC of asthmatic animal plasma was low as 2.132 ± 0.986 mM Trolox Eq/L (p < 0.009).
The level of these biomarkers reverts back towards normal after treatment with marketed and developed
formulation, although treatment with developed formulation was more efficacious since it was coformulated
with GTE, which acts as an adjuvant for the management of inflammatory disease like asthma.
Conclusion: It is contemplated that, use of GTE as an adjuvant to anti leukotriene drug played a significant
role in asthma management by reducing oxidant injury. Since, studies in animals do not directly
translate to human biology, further multi-control studies with better sampled patient population
and more number of patients are needed.