Background: Phytochemical studies of Annona muricata showed the presence of bioactive
components with anticancer activity. We compared the anticancer properties of crude acetonic
and methanolic A. muricata leaf extracts.
Methods: The viabilities of different cell lines (A549, U87, U251, K562 and VERO) treated with A.
muricata acetonic or methanolic leaf extracts were measured using the MTT assay. Apoptosis induction,
cell cycle and cytoskeleton rearrangements were evaluated in K562 by flow cytometry or
Results: Chemical analyses of the A. muricata extracts showed differences in their composition. The
K562 cell line was the most sensitive to the treatment with the acetonic and methanolic extracts, and
the IC50 values, respectively were 28.82 (24.41 - 34.69) and 32.49 (27.21 - 40.16) μg/mL. Both extracts
induced apoptotic cell death and G0/G1 phase cell cycle arrest. For the first time, cytoskeleton
rearrangements were observed in the K562 cell line treated with methanolic extract.
Conclusion: These findings suggest that both A. muricata extracts exhibit antileukemic potential
and represent a promising source of novel compounds with anticancer activity.