Expression and Purification of Tag-removed Human IL37 by Digestion on Beads in Escherichia coli

Author(s): Daichao Wu, Guoqing Li, Yao Ma, Jin Liu, Yukun Li, Juan Wang, Junhui Bai, Sijie Tan*, Meixiang Li*, Yongheng Chen*.

Journal Name: Protein & Peptide Letters

Volume 25 , Issue 11 , 2018

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Graphical Abstract:


Background: Human Interleukin 37 (IL37), a unique anti-inflammatory cytokine of IL1 family member, plays critical roles in innate and adaptive immunity and inflammation.

Objective: Preparation of high purity and tag-removed recombinant IL37 protein (rIL37) is critical for its clinical application.

Method: In this study, we constructed an N-terminal cleavable GST-fused IL37 expression vector for recombinant expression.

Results: Subsequent to transformation and optimization of the induction temperature, the soluble expression level of rIL37 was 306.5 mg/L of culture medium at 18 °C induction in Escherichia coli. Meanwhile, rIL37 was digested on beads by GST-HRV3C protease during GST affinity chromatography. After further purification, the purity of rIL37 was higher than 99 %. Finally, the antiinflammatory activity of tag-removed protein was verified by the results showing that rIL37 suppressed IL1β production in PBMCs.

Conclusion: This work presents a protocol to produce high purity and tag-removed rIL37 with antiinflammatory activity, which provides the firm basis for advancing clinical application in human IL37-related inflammatory diseases.

Keywords: Soluble expression, purification, interleukin 37, Escherichia coli, digestion on beads, inflammatory diseases.

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Article Details

Year: 2018
Page: [996 - 1002]
Pages: 7
DOI: 10.2174/0929866526666181128124028
Price: $58

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