Background: Chronic inflammation is a critical health issue and implicated in
several chronic health problems such as tumors, auto-immune disorder, hypertension or diabetes.
However, Juniperus procera is one of the famous ancient plants that has been traditionally
used to treat several diseases such as hyperglycemia, hepatitis, jaundice, bronchitis,
Objective: Current study is an attempt to investigate the anti-inflammatory effect of Juniperus
procera extract on rats exposed to cytotoxicity caused experimentally by streptozotocin
Methods: Five groups of adult Wistar rats (10 rats each) were examined as (Normal control,
Normal rats treated with Juniperus procera extract, rats administrated with streptozotocin,
rats administrated with streptozotocin and treated with insulin and, rats administrated with
streptozotocin and Juniperus procera extract). At the end of the experiment, blood was
collected from experimented rats. Animals then were killed and small parts of both pancreas
and liver were collected for gene expression and histopathological examination.
Results: Serum analysis showed a significant increase in glucose, IL-6, IL-2 and TNF-α
levels in rats exposed to streptozotocin. That change was reduced in rats cotreated with insulin
or Juniperus procera extract. Moreover, streptozotocin showed a significant upregulation
of IL-6, TNF-α and A2M genes, while, either insulin or Juniperus procera treatment
was restored to normal status. Streptozotocin induced inflammation within hepatic tissues
which clearly reduced in hepatic tissues of both insulin and junipers cotreated groups.
Conclusion: Streptozotocin toxicity induces acute inflammation and increases serum glucose,
IL-6, IL-2 and TNF-α levels. However, Juniperus procera extract was found to significantly
prevent that reaction within four weeks experimented frame time.